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CoraLite® Plus 488-conjugated HSP90 Monoclonal antibody

HSP90 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

IF, FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3F11C1

Cat no : CL488-60318

Synonyms

FLJ31884, Heat shock 86 kDa, HSP 86, HSP86, Hsp89, HSP89A, HSP90, HSP90A, HSP90AA1, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, LAP2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated HSP90 Monoclonal ant (CL488-60318)

    IF Staining of HepG2 using CL488-60318

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-60318 (HSP90 antibody) at dilution of 1:100.

Tested Applications

Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

FCSee 1 publications below

Product Information

CL488-60318 targets HSP90 in IF, FC applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HSP90 fusion protein Ag3826
Full Name heat shock protein 90kDa alpha (cytosolic), class A member 1
Calculated Molecular Weight 853 aa, 90 kDa
Observed Molecular Weight 85-90 kDa
GenBank Accession NumberBC023006
Gene Symbol HSP90AA1
Gene ID (NCBI) 3320
RRIDAB_2883143
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSP90, encoded by HSP90AA1, is a constitutively and ubiquitously expressed molecular chaperone that is crucial for the stability and function of many proteins. HSP90 provides chaperoning activity for client proteins; many of them are members of oncogenic pathways, indicating its implication in tumor malignancy. HSP90 mainly resides in the cytosol, while it can also be released to the extracellular space. Secreted Hsp90 is a C-terminal truncated form. It has been reported that the level of plasma Hsp90 is positively correlated with tumor malignancy in clinical cancer patients, and can be a promising diagnostic marker for tumor malignancy in clinical application.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 HSP90 antibody CL488-60318Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanFC

J Mater Chem B

Magnetic hyperthermia induces effective and genuine immunogenic tumor cell death with respect to exogenous heating

Authors - Bin Yan
View Article