CoraLite®594-conjugated HSP90 Monoclonal antibody

HSP90 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3F11C1

Cat no : CL594-60318

Synonyms

FLJ31884, Heat shock 86 kDa, HSP 86, HSP86, Hsp89, HSP89A, HSP90, HSP90A, HSP90AA1, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, LAP2



Tested Applications

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 60318-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-60318 targets HSP90 in IF applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HSP90 fusion protein Ag3826
Full Name heat shock protein 90kDa alpha (cytosolic), class A member 1
Calculated molecular weight 853 aa, 90 kDa
Observed molecular weight 85-90 kDa
GenBank accession numberBC023006
Gene symbol HSP90AA1
Gene ID (NCBI) 3320
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSP90, encoded by HSP90AA1, is a constitutively and ubiquitously expressed molecular chaperone that is crucial for the stability and function of many proteins. HSP90 provides chaperoning activity for client proteins; many of them are members of oncogenic pathways, indicating its implication in tumor malignancy. HSP90 mainly resides in the cytosol, while it can also be released to the extracellular space. Secreted Hsp90 is a C-terminal truncated form. It has been reported that the level of plasma Hsp90 is positively correlated with tumor malignancy in clinical cancer patients, and can be a promising diagnostic marker for tumor malignancy in clinical application.

Protocols

Product Specific Protocols
IF protocol for CL594 HSP90 antibody CL594-60318Download protocol
Standard Protocols
Click here to view our Standard Protocols