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GRP94 Polyclonal antibody

GRP94 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 14700-1-AP

Synonyms

ECGP, Endoplasmin, GP96, gp96 homolog, GRP 94, GRP94, HSP90B1, TRA1, Tumor rejection antigen 1

Validation Data Gallery

  • WB analysis of HeLa using 14700-1-AP

    WB analysis of HeLa using 14700-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14700-1-AP (HSP90B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 14700-1-AP

    IHC staining of human breast cancer using 14700-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14700-1-AP (HSP90B1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human breast cancer using 14700-1-AP

    IHC staining of human breast cancer using 14700-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14700-1-AP (HSP90B1 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of A431 using 14700-1-AP

    IF Staining of A431 using 14700-1-AP

    Immunofluorescent analysis of A431 cells, using HSP90B1 antibody 14700-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IP experiment of mouse liver using 14700-1-AP

    IP experiment of mouse liver using 14700-1-AP

    IP Result of anti-GRP94 (IP:14700-1-AP, 3ug; Detection:14700-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

  • FC experiment of HEK-293T using 14700-1-AP

    FC experiment of HEK-293T using 14700-1-AP

    1X10^6 HEK-293T cells were stained with .2ug GRP94 antibody (14700-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inHeLa cells, A549 cells, HEK-293 cells, HT-1080 cells, K-562 cells, MCF-7 cells, mouse heart tissue, mouse liver tissue, NIH/3T3 cells, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman breast cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells, HepG2 cells
Positive FC detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14700-1-AP targets GRP94 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRP94 fusion protein Ag6344
Full Name heat shock protein 90kDa beta (Grp94), member 1
Calculated molecular weight 92 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC066656
Gene symbol HSP90B1
Gene ID (NCBI) 7184
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSP90 proteins are highly conserved molecular chaperones, which normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 (GP96 or GRP94) is an endoplasmic reticulum paralogue of the cytosolic HSP90. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors (TLRs), HSP90b1 chaperones peptides to MHC class I molecules of dendritic cells and other antigen-presenting cells, as well as facilitating the assembly of immunoglobulin. The protein is also involved in many other bio-processes. This antibody was generated against the C-terminal region of full-length HSP90b1.

Protocols

Product Specific Protocols
WB protocol for GRP94 antibody 14700-1-APDownload protocol
IHC protocol for GRP94 antibody 14700-1-APDownload protocol
IF protocol for GRP94 antibody 14700-1-APDownload protocol
IP protocol for GRP94 antibody 14700-1-APDownload protocol
FC protocol for GRP94 antibody 14700-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Chem Res Toxicol

Polychlorinated biphenyl quinone induces endoplasmic reticulum stress, unfolded protein response and calcium release.

Authors - Demei Xu
View Article
humanWB

Sci Adv

Platelet P-selectin initiates cross-presentation and dendritic cell differentiation in blood monocytes.

Authors - Patrick Han
View Article
humanWB

Exp Ther Med

PCSK9 regulates apoptosis in human lung adenocarcinoma A549 cells via endoplasmic reticulum stress and mitochondrial signaling pathways.

Authors - Xiaohui Xu
View Article
ratWB

Int J Mol Med

Hydrogen sulfide attenuates myocardial fibrosis in diabetic rats through the JAK/STAT signaling pathway.

Authors - Maojun Liu
View Article
humanWB

Int J Mol Sci

Upregulation of Phosphorylated HSP27, PRDX2, GRP75, GRP78 and GRP94 in Acquired Middle Ear Cholesteatoma Growth.

Authors - Ho Kuen Yao KY
View Article
humanWB

Drug Des Devel Ther

PACE4 regulates apoptosis in human prostate cancer cells via endoplasmic reticulum stress and mitochondrial signaling pathways.

Authors - Zhiyong Yao
View Article