A549 cells were subjected to SDS PAGE followed by western blot with 14700-1-AP(HSP90B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

HEK-293 cells were subjected to SDS PAGE followed by western blot with 14700-1-AP(HSP90B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 14700-1-AP(HSP90B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

HT-1080 cells were subjected to SDS PAGE followed by western blot with 14700-1-AP(HSP90B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

K-562 cells were subjected to SDS PAGE followed by western blot with 14700-1-AP(HSP90B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

MCF7 cells were subjected to SDS PAGE followed by western blot with 14700-1-AP(HSP90B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

mouse heart tissue were subjected to SDS PAGE followed by western blot with 14700-1-AP(HSP90B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

mouse liver tissue were subjected to SDS PAGE followed by western blot with 14700-1-AP( GRP94 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14700-1-AP(HSP90B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

rat liver tissue were subjected to SDS PAGE followed by western blot with 14700-1-AP( GRP94 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

Immunohistochemical analysis of paraffin-embedded human breast cancer using 14700-1-AP(HSP90B1 antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical analysis of paraffin-embedded human breast cancer using 14700-1-AP(HSP90B1 antibody) at dilution of 1:100 (under 40x lens)

Immunohistochemical analysis of paraffin-embedded human cervical cancer slide using 14700-1-AP( HSP90B1 Antibody) at dilution of 1:50

Immunohistochemical analysis of paraffin-embedded human cervical cancer slide using 14700-1-AP( HSP90B1 Antibody) at dilution of 1:50

IP Result of anti-GRP94 (IP:14700-1-AP, 3ug; Detection:14700-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

Immunofluorescent analysis of A431 cells, using HSP90B1 antibody 14700-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunofluorescent analysis of HepG2 cells, using HSP90B1 antibody 14700-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

1X10^6 HEK-293T cells were stained with .2ug GRP94 antibody (14700-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.