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GRP94 Antibody 12 Publications

Rabbit Polyclonal| Catalog number: 14700-1-AP

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Con: 36 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

GRP94 Antibody WB HeLa cells 14700-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 14700-1-AP(HSP90B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

GRP94 Antibody IHC human breast cancer tissue 14700-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer using 14700-1-AP(HSP90B1 antibody) at dilution of 1:100 (under 10x lens)

GRP94 Antibody IHC human breast cancer tissue 14700-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer using 14700-1-AP(HSP90B1 antibody) at dilution of 1:100 (under 40x lens)

GRP94 Antibody IF A431 cells 14700-1-AP

Immunofluorescent analysis of A431 cells, using HSP90B1 antibody 14700-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

GRP94 Antibody IP mouse liver tissue 14700-1-AP

IP Result of anti-GRP94 (IP:14700-1-AP, 3ug; Detection:14700-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

GRP94 Antibody FC HEK-293T cells 14700-1-AP

1X10^6 HEK-293T cells were stained with .2ug GRP94 antibody (14700-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, mouse, rat
Positive WB detected in:
HeLa cells, A549 cells, HEK-293 cells, HT-1080 cells, K-562 cells, MCF-7 cells, mouse heart tissue, mouse liver tissue, NIH/3T3 cells, rat liver tissue
Positive IP detected in:
mouse liver tissue
Positive IHC detected in:
human breast cancer tissue, human cervical cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
A431 cells, HepG2 cells
Positive FC detected in:
HEK-293T cells
Recommended dilution:
WB : 1:1000-1:4000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
GRP94 fusion protein Ag6344
Full name:
heat shock protein 90kDa beta (Grp94), member 1
Calculated molecular weight:
92 kDa
Observed molecular weight:
100 kDa
GenBank accession number:
BC066656
Gene ID (NCBI):
7184
Gene symbol
HSP90B1
Synonyms
ECGP, Endoplasmin, GP96, gp96 homolog, GRP 94, GRP94, HSP90B1, TRA1, Tumor rejection antigen 1

Background

HSP90 proteins are highly conserved molecular chaperones, which normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 (GP96 or GRP94) is an endoplasmic reticulum paralogue of the cytosolic HSP90. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors (TLRs), HSP90b1 chaperones peptides to MHC class I molecules of dendritic cells and other antigen-presenting cells, as well as facilitating the assembly of immunoglobulin. The protein is also involved in many other bio-processes. This antibody was generated against the C-terminal region of full-length HSP90b1.


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