AuthentiKine™ Human HGF ELISA Kit

Sensitivity

0.26 ng/mL

Range

0.313-20 ng/mL

Reacted Species

Human

Cat no : KE00168

Synonyms

F TCF, Hepatocyte growth factor, Hepatopoeitin A, HGF, HGFB, HPTA, Scatter factor, SF



Product Information

KE00168 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The HGF ELISA kit is to be used to detect and quantify protein levels of endogenous HGF. The assay recognizes human HGF. An antibody specific for HGF has been pre-coated onto the microwells. The HGF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for HGF is added to detect the captured HGF protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Product name AuthentiKine™ Human HGF ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 0.26 ng/mL
Range 0.313-20 ng/mL
Reacted Species Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Human Serum91%73%-112%
Human plasma91%73%-112%
Cell culture supernatants117%93%-126%

IntraAssay

Samplenmean ( ng/mL)SDCV%
1204.70.306.4
2209.30.657.0
32018.30.925.0

InterAssay

Samplenmean ( ng/mL)SDCV%
1245.10.428.4
22410.50.969.2
32420.919.39.2

Background Information

Hepatocyte growth factor (HGF) is the most potent mitogen of mature hepatocytes in primary culture. HGF is derived from a biologically inactive single chain precursor of 728 amino acids (pro-HGF) localized mostly on the cell surface and in the extracellular matrix. The mature form produced following proteolytic cleavage is composed of a 69-kDa alpha-subunit (containing four kringle domains) and the 34 kDa beta-subunit, similar to the catalytic domain of serine proteases, but with amino acid substitutions in the active site. HGF is a pleiotropic cytokine which exerts a variety of effects on several cells, being involved in the regulation of many biological processes, such as inflammation, tissue repair, morphogenesis, angiogenesis, tumour propagation, immunomodulation of viral infections and cardio-metabolic activities.

Properties

Storage Instructions All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms F TCF, Hepatocyte growth factor, Hepatopoeitin A, HGF, HGFB, HPTA, Scatter factor, SF