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IARS2 Antibody

IARS2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17170-1-AP

Synonyms

IARS2, IleRS, Isoleucine tRNA ligase



Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, mouse lung tissue, Jurkat cells
Positive IP detected inHeLa cells, Jurkat cells
Positive IHC detected inhuman liver cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

17170-1-AP targets IARS2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IARS2 fusion protein Ag10696
Full Name isoleucyl-tRNA synthetase 2, mitochondrial
Calculated molecular weight 1012aa,114 kDa
Observed molecular weight 114 kDa
GenBank accession number BC010218
Gene symbol IARS2
Gene ID (NCBI) 55699
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IARS2 antibody 17170-1-APDownload protocol
IHC protocol for IARS2 antibody 17170-1-APDownload protocol
IF protocol for IARS2 antibody 17170-1-APDownload protocol
IP protocol for IARS2 antibody 17170-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Oncol

The Oncogene IARS2 Promotes Non-small Cell Lung Cancer Tumorigenesis by Activating the AKT/MTOR Pathway.

Authors - Xin Di
  • KD Validated