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  • KD/KO Validated

IDO1 Polyclonal antibody

IDO1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13268-1-AP

Synonyms

CD107B, IDO, IDO 1, IDO1, INDO, indoleamine 2,3 dioxygenase 1, Indoleamine 2,3-dioxygenase 1, Indoleamine-pyrrole 2,3-dioxygenase



Tested Applications

Positive WB detected inhuman placenta tissue, IFN gamma treated HeLa cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13268-1-AP targets IDO1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IDO1 fusion protein Ag3953
Full Name indoleamine 2,3-dioxygenase 1
Calculated molecular weight 403 aa, 45 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC027882
Gene symbol IDO1
Gene ID (NCBI) 3620
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IDO1 is the target for therapy in a range of clinical settings, including cancer, chronic infections, autoimmune and allergic syndromes, and transplantation. Elevated IDO1 expression is a hallmark of major viral infections including HIV, HBV, HCV or influenza and also of major bacteria infections, such as Tb, CAP, listeriosis and sepsis. Pathogens are able to highjack the immunosuppressive effects of IDO1 and make use of them to facilitate their own life cycle. MW of IDO1 is 40-42kd (PMID: 14502282; 17055065).

Protocols

Product Specific Protocols
WB protocol for IDO1 antibody 13268-1-APDownload protocol
IHC protocol for IDO1 antibody 13268-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cancer Lett

Multiple myeloma cell-derived IL-32γ increases the immunosuppressive function of macrophages by promoting indoleamine 2,3-dioxygenase (IDO) expression.

Authors - Haimeng Yan
humanWB

Front Cell Infect Microbiol

Toxoplasma Effector GRA15-Dependent Suppression of IFN-γ-Induced Antiparasitic Response in Human Neurons.

Authors - Hironori Bando
ratWB

Int Immunopharmacol

Curcumin relieves depressive-like behaviors via inhibition of the NLRP3 inflammasome and kynurenine pathway in rats suffering from chronic unpredictable mild stress.

Authors - Wen-Yuan Zhang
humanWB, IF

J Oral Pathol Med

Interferon-γ regulates the function of mesenchymal stem cells from oral lichen planus via indoleamine 2,3-dioxygenase activity.

Authors - Zhihui Zhang
humanWB

Biochem Biophys Res Commun

Nrf2 modulates immunosuppressive ability and cellular senescence of human umbilical cord mesenchymal stem cells.

Authors - Zhang Jie
ratWB

Eur J Nutr

Fish oil supplementation attenuates neuroinflammation and alleviates depressive-like behavior in rats submitted to repeated lipopolysaccharide.

Authors - Ruili Dang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Hala (Verified Customer) (09-18-2020)

I am very satisfied of this antibody and of the interaction with your company

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: macrophages and hela cells
IDO1 Antibody Western Blot validation (1:1000 dilution) in macrophages and hela cells (Cat no:13268-1-AP)