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IFT57 Polyclonal antibody
IFT57 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Cat no : 11083-1-AP
|Positive WB detected in||mouse retina tissue, HEK-293 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human prostate cancer tissue, human pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MDCK cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11083-1-AP targets IFT57 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat, Canine samples.
|Tested Reactivity||human, mouse, rat, Canine|
|Cited Reactivity||Chicken, human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IFT57 fusion protein Ag1566|
|Full Name||intraflagellar transport 57 homolog (Chlamydomonas)|
|Calculated molecular weight||49 kDa|
|Observed molecular weight||49 kDa|
|GenBank accession number||BC011899|
|Gene ID (NCBI)||55081|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
IFT57, also named as DERP8, ESRRBL1, HIPPI and MHS4R2, belongs to the IFT57 family. It is required for the formation of cilia. IFT57 plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway. It is a component of IFT complex B which composed of IFT88, IFT57, TRAF3IP1, IFT52, IFT27, HSPB11 and IFT20. In contrast to BBS proteins, IFT57 and IFT88 is found in almost every cilium.(PMID:22072986)
|Product Specific Protocols|
|WB protocol for IFT57 antibody 11083-1-AP||Download protocol|
|IHC protocol for IFT57 antibody 11083-1-AP||Download protocol|
|IF protocol for IFT57 antibody 11083-1-AP||Download protocol|
|IP protocol for IFT57 antibody 11083-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Hum Mol Genet
Human IFT52 mutations uncover a novel role for the protein in microtubule dynamics and centrosome cohesion.
DYNC2LI1 mutations broaden the clinical spectrum of dynein-2 defects.
Characterization of tetratricopeptide repeat-containing proteins critical for cilia formation and function.
Mol Biol Cell
Cooperation of the IFT-A complex with the IFT-B complex is required for ciliary retrograde protein trafficking and GPCR import.
J Biol Chem
Overall architecture of the intraflagellar transport (IFT)-B complex containing Cluap1/IFT38 as an essential component of the IFT-B peripheral subcomplex.
Chibby functions to preserve normal ciliary morphology through the regulation of intraflagellar transport in airway ciliated cells.