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  • KD/KO Validated

IGF2BP3 Polyclonal antibody

IGF2BP3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14642-1-AP

Synonyms

hKOC, IGF II mRNA binding protein 3, IGF2 mRNA binding protein 3, IGF2BP3, IMP 3, IMP3, KOC1, VICKZ family member 3, VICKZ3



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, BxPC-3 cells, mouse brain tissue, HepG2 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

14642-1-AP targets IGF2BP3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IGF2BP3 fusion protein Ag6226
Full Name insulin-like growth factor 2 mRNA binding protein 3
Calculated molecular weight 64 kDa
Observed molecular weight 64 kDa
GenBank accession numberBC065269
Gene symbol IGF2BP3
Gene ID (NCBI) 10643
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IGF2BP3, also named as IMP3, KOC1 and VICKZ3, belongs to the RRM IMP/VICKZ family. It is one of the RNA binding proteins involved in mRNA localization and translational control. IGF2BP3 is expressed during embryogenesis, as well as in some malignant tumors. It can be used as an independent prognostic factor for osteosarcoma. Both isoforms (64kd and 22kd) of IGFBP3 can be recognized by this antibody. And IGFBP3 is nuclear and cytoplasm stains.

Protocols

Product Specific Protocols
WB protocol for IGF2BP3 antibody 14642-1-APDownload protocol
IHC protocol for IGF2BP3 antibody 14642-1-APDownload protocol
IF protocol for IGF2BP3 antibody 14642-1-APDownload protocol
IP protocol for IGF2BP3 antibody 14642-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Physiol

FNDC3B circular RNA promotes the migration and invasion of gastric cancer cells via the regulation of E-cadherin and CD44 expression.

Authors - Yuling Hong
humanWB

J Cell Mol Med

Wilms' tumour 1-associating protein inhibits endothelial cell angiogenesis by m6A-dependent epigenetic silencing of desmoplakin in brain arteriovenous malformation.

Authors - Lin-Jian Wang
  • KD Validated
humanWB

Int J Mol Sci

EIF4G1 and RAN as Possible Drivers for Malignant Pleural Mesothelioma.

Authors - Irene Dell'Anno
  • KD Validated
humanIHC

Oncoscience

Expression of the cancer testis antigen IGF2BP3 in colorectal cancers; IGF2BP3 holds promise as a specific immunotherapy target.

Authors - Hmc Shantha Kumara
humanWB,IHC

J Exp Clin Cancer Res

RNA N6-methyladenosine reader IGF2BP3 regulates cell cycle and angiogenesis in colon cancer.

Authors - Zhou Yang
humanIHC

J Mol Histol

The distribution of IGF2 and IMP3 in osteosarcoma and its relationship with angiogenesis.

Authors - Chen Peng P

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jessica (Verified Customer) (10-29-2018)

Also binds exogenous expressed IGF2BP1-GFP protein. I also get a double band around 70 kDa that is likely IGF2BP1 and IGF2BP3. This antibody is not specific.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1500
  • Cell Tissue Type: Neuroblastoma cells (human)