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  • KD/KO Validated

IGF2BP3 Antibody

IGF2BP3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat no : 14642-1-AP

Synonyms

hKOC, IGF II mRNA binding protein 3, IGF2 mRNA binding protein 3, IGF2BP3, IMP 3, IMP3, KOC1, VICKZ family member 3, VICKZ3



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, BxPC-3 cells, mouse brain tissue, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman pancreas tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

14642-1-AP targets IGF2BP3 in IF, IHC, IP, WB, ELISA applications and shows reactivity with human, mouse samples.

Reactivity human, mouse
Cited Species human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IGF2BP3 fusion protein Ag6226
Full Name insulin-like growth factor 2 mRNA binding protein 3
Calculated molecular weight 64 kDa
Observed molecular weight 64 kDa
GenBank accession number BC065269
Gene symbol IGF2BP3
Gene ID (NCBI) 10643
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

IGF2BP3, also named as IMP3, KOC1 and VICKZ3, belongs to the RRM IMP/VICKZ family. It is one of the RNA binding proteins involved in mRNA localization and translational control. IGF2BP3 is expressed during embryogenesis, as well as in some malignant tumors. It can be used as an independent prognostic factor for osteosarcoma. Both isoforms (64kd and 22kd) of IGFBP3 can be recognized by this antibody. And IGFBP3 is nuclear and cytoplasm stains.

Protocols

Product Specific Protocols
WB protocol for IGF2BP3 antibody 14642-1-APDownload protocol
IHC protocol for IGF2BP3 antibody 14642-1-APDownload protocol
IF protocol for IGF2BP3 antibody 14642-1-APDownload protocol
IP protocol for IGF2BP3 antibody 14642-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Physiol

FNDC3B circular RNA promotes the migration and invasion of gastric cancer cells via the regulation of E-cadherin and CD44 expression.

Authors - Yuling Hong
humanWB

J Cell Mol Med

Wilms' tumour 1-associating protein inhibits endothelial cell angiogenesis by m6A-dependent epigenetic silencing of desmoplakin in brain arteriovenous malformation.

Authors - Lin-Jian Wang
  • KD Validated
humanIHC

Oncoscience

Expression of the cancer testis antigen IGF2BP3 in colorectal cancers; IGF2BP3 holds promise as a specific immunotherapy target.

Authors - Hmc Shantha Kumara
humanIHC

J Mol Histol

The distribution of IGF2 and IMP3 in osteosarcoma and its relationship with angiogenesis.

Authors - Chen Peng P
humanWB

Biochem Biophys Res Commun

Ubiquitination of IGF2BP3 by E3 ligase MKRN2 regulates the proliferation and migration of human neuroblastoma SHSY5Y cells.

Authors - Caiwei Jia
ratWB

Oncotarget

Treatment of diabetes mellitus-induced erectile dysfunction using endothelial progenitor cells genetically modified with human telomerase reverse transcriptase.

Authors - Yan Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jessica Bell (10-29-2018)

Also binds exogenous expressed IGF2BP1-GFP protein. I also get a double band around 70 kDa that is likely IGF2BP1 and IGF2BP3. This antibody is not specific.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1500
  • Cell Tissue Type: Neuroblastoma cells (human)
No image submitted with review