- Featured Product
- KD/KO Validated
IGF2BP3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 14642-1-AP
hKOC, IGF II mRNA binding protein 3, IGF2 mRNA binding protein 3, IGF2BP3, IMP 3, IMP3, KOC1, VICKZ family member 3, VICKZ3
|Positive WB detected in||HEK-293 cells, HeLa cells, BxPC-3 cells, mouse brain tissue, HepG2 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
14642-1-AP targets IGF2BP3 in IF, IHC, IP, WB, ELISA applications and shows reactivity with human, mouse samples.
|Cited Species||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IGF2BP3 fusion protein Ag6226|
|Full Name||insulin-like growth factor 2 mRNA binding protein 3|
|Calculated molecular weight||64 kDa|
|Observed molecular weight||64 kDa|
|GenBank accession number||BC065269|
|Gene ID (NCBI)||10643|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20oC. Aliquoting is unnecessary for -20oC storage.|
IGF2BP3, also named as IMP3, KOC1 and VICKZ3, belongs to the RRM IMP/VICKZ family. It is one of the RNA binding proteins involved in mRNA localization and translational control. IGF2BP3 is expressed during embryogenesis, as well as in some malignant tumors. It can be used as an independent prognostic factor for osteosarcoma. Both isoforms (64kd and 22kd) of IGFBP3 can be recognized by this antibody. And IGFBP3 is nuclear and cytoplasm stains.
|Product Specific Protocols|
|WB protocol for IGF2BP3 antibody 14642-1-AP||Download protocol|
|IHC protocol for IGF2BP3 antibody 14642-1-AP||Download protocol|
|IF protocol for IGF2BP3 antibody 14642-1-AP||Download protocol|
|IP protocol for IGF2BP3 antibody 14642-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Cell Physiol
FNDC3B circular RNA promotes the migration and invasion of gastric cancer cells via the regulation of E-cadherin and CD44 expression.
J Cell Mol Med
Wilms' tumour 1-associating protein inhibits endothelial cell angiogenesis by m6A-dependent epigenetic silencing of desmoplakin in brain arteriovenous malformation.
Expression of the cancer testis antigen IGF2BP3 in colorectal cancers; IGF2BP3 holds promise as a specific immunotherapy target.
J Mol Histol
The distribution of IGF2 and IMP3 in osteosarcoma and its relationship with angiogenesis.
Biochem Biophys Res Commun
Ubiquitination of IGF2BP3 by E3 ligase MKRN2 regulates the proliferation and migration of human neuroblastoma SHSY5Y cells.
Treatment of diabetes mellitus-induced erectile dysfunction using endothelial progenitor cells genetically modified with human telomerase reverse transcriptase.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Jessica Bell (10-29-2018)
Also binds exogenous expressed IGF2BP1-GFP protein. I also get a double band around 70 kDa that is likely IGF2BP1 and IGF2BP3. This antibody is not specific.