IL-12B Polyclonal antibody

IL-12B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 17645-1-AP

Synonyms

CLMF, CLMF p40, CLMF2, IL 12 subunit p40, IL 12B, IL12B, IL-12B, Interleukin 12 subunit beta, NKSF, NKSF2



Tested Applications

Positive WB detected inRecombinant protein, Recombinant protein protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 17645-1-AP is IL-12B Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-12B fusion protein Ag11837
Full Name interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
Calculated molecular weight 328 aa, 37 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC067498
Gene symbol IL12B
Gene ID (NCBI) 3593
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin-12 (IL-12), is a cytokine that is secreted by activated phagocytes and dendritic cells and that induces interferon-γproduction by natural-killer and T lymphocytes. IL-12 is a 75 kDa heterodimer composed of a 35 kDa subunit (IL-12A p35) and a 40 kDa subunit (IL-12B p40) that is secreted by a wide variety of antigen presenting cells (APCs), including phagocytes, B cells and Langerhans cells. IL-12B has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of IL-12B was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of IL-12B has been reported to be associated with the severity of atopic and non-atopic asthma in children.

Protocols

Product Specific Protocols
WB protocol for IL-12B antibody 17645-1-APDownload protocol
Standard Protocols
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