IL-1 Beta Monoclonal antibody

IL-1 Beta Monoclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Mouse / IgG1


human and More (2)







Cat no : 60136-1-Ig


Tested Applications

Positive WB detected inRecombinant protein
Positive IP detected inRAW 264.7 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60136-1-Ig targets IL-1 Beta in WB, IP, IHC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IL-1 Beta fusion protein Ag10295
Full Name interleukin 1, beta
Calculated molecular weight 269 aa, 31 kDa
GenBank accession numberBC008678
Gene symbol IL1B
Gene ID (NCBI) 3553
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15 to 18 kDa protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets. The expression level of IL-1B is very low.


Product Specific Protocols
WB protocol for IL-1 Beta antibody 60136-1-IgDownload protocol
IHC protocol for IL-1 Beta antibody 60136-1-IgDownload protocol
IP protocol for IL-1 Beta antibody 60136-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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