IL-1 Beta Monoclonal antibody

IL-1 Beta Monoclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (2)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C6F12

Cat no : 60136-1-Ig

Synonyms



Tested Applications

Positive WB detected inRecombinant protein
Positive IP detected inRAW 264.7 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60136-1-Ig targets IL-1 Beta in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IL-1 Beta fusion protein Ag10295
Full Name interleukin 1, beta
Calculated molecular weight 269 aa, 31 kDa
GenBank accession numberBC008678
Gene symbol IL1B
Gene ID (NCBI) 3553
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15 to 18 kDa protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets. The expression level of IL-1B is very low.

Protocols

Product Specific Protocols
WB protocol for IL-1 Beta antibody 60136-1-IgDownload protocol
IHC protocol for IL-1 Beta antibody 60136-1-IgDownload protocol
IP protocol for IL-1 Beta antibody 60136-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanELISA

Food Funct

Pepsin-pancreatin hydrolysis reduced the ability of lunasin-enriched material to inhibit activation of the inflammasomes in THP-1 human macrophages.

Authors - Samuel James Price
humanWB

Placenta

PMID: 26311557

Authors - Chuan Wang
ratWB

Cardiovasc Diabetol

Anti-inflammatory effects of triptolide improve left ventricular function in a rat model of diabetic cardiomyopathy.

Authors - Wen He-Ling HL
humanWB

Cell Commun Signal

A novel protein derived from lamprey supraneural body tissue with efficient cytocidal actions against tumor cells.

Authors - Yue Pang
ratWB

J Neuroinflammation

Protection of ischemic post conditioning against transient focal ischemia-induced brain damage is associated with inhibition of neuroinflammation via modulation of TLR2 and TLR4 pathways.

Authors - Ying Wang
humanWB

Oncotarget

Loss of AIM2 expression promotes hepatocarcinoma progression through activation of mTOR-S6K1 pathway.

Authors - Xiaomin Ma