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IL-20RB Polyclonal antibody

IL-20RB Polyclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 20521-1-AP

Synonyms

DIRS1, FNDC6, IL 20 receptor subunit beta, IL 20R beta, IL 20R2, IL 20RB, IL20RB, IL-20RB, interleukin 20 receptor beta

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using IL-20RB Polyclonal antibody (20521-1-AP)

    WB analysis of MCF-7 using 20521-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 20521-1-AP (IL20RB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using IL-20RB Polyclonal antibody (20521-1-AP)

    WB analysis of HeLa using 20521-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20521-1-AP (IL20RB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using IL-20RB Polyclonal antibody (20521-1-AP)

    WB analysis of K-562 using 20521-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 20521-1-AP (IL20RB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Apoptosised HeLa cells using IL-20RB Polyclonal antibody (20521-1-AP)

    WB analysis of Apoptosised HeLa using 20521-1-AP

    Apoptosised HeLa cells were subjected to SDS PAGE followed by western blot with 20521-1-AP (IL20RB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using IL-20RB Polyclonal antibody (20521-1-AP)

    IF Staining of HepG2 using 20521-1-AP

    Immunofluorescent analysis of HepG2 cells, using IL20RB antibody 20521-1-AP at 1:25 dilution and FITC- labeled goat anti-Rabbit IgG (green).

  • Flow cytometry (FC) experiment of K-562 cells using IL-20RB Polyclonal antibody (20521-1-AP)

    FC experiment of K-562 using 20521-1-AP

    1X10^6 K-562 cells were stained with 0.2ug IL20RB antibody (20521-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inMCF7 cells, Apoptosised HeLa cells, HeLa cells, K-562 cells
Positive IF detected inHepG2 cells
Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20521-1-AP is IL-20RB Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-20RB fusion protein Ag14368
Full Name interleukin 20 receptor beta
Calculated Molecular Weight 311 aa, 35 kDa
Observed Molecular Weight 45 kDa, 66-70 kDa
GenBank Accession NumberBC033292
Gene Symbol IL20RB
Gene ID (NCBI) 53833
RRIDAB_10700002
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-20 receptor subunit beta (IL20RB) is a single-pass type I membrane protein of the type II cytokine receptor family. The interleukin-20-receptor I complex (IL-20-RI) is composed of two chains, IL20RA and IL20RB. IL-20-RI is a receptor for IL-19, IL-20 and IL-24. IL20RB can also form a heterodimer with IL22RA1. The IL22RA1/IL20RB dimer is a receptor for IL20 and IL24.

Protocols

Product Specific Protocols
WB protocol for IL-20RB antibody 20521-1-APDownload protocol
IF protocol for IL-20RB antibody 20521-1-APDownload protocol
FC protocol for IL-20RB antibody 20521-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanFC

Front Immunol

Integrative single-cell transcriptomic investigation unveils long non-coding RNAs associated with localized cellular inflammation in psoriasis

Authors - Yuge Gao
View Article
human,mouseWB,IF

J Transl Med

IL20RB signaling enhances stemness and chemotherapy resistance in pancreatic cancer

Authors - Xiao-Hui Li
View Article