IL-33 Monoclonal antibody

IL-33 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2A9C7

Cat no : 66235-1-Ig

Synonyms

C9orf26, DVS27, IL 1F11, IL 33, IL1F11, IL33, IL-33, Interleukin 1 family member 11, interleukin 33, NF HEV, NFEHEV, NFHEV, RP11 575C20.2



Tested Applications

Positive WB detected inhuman lung tissue, HepG2 cells
Positive IHC detected inhuman nasopharyngeal carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66235-1-Ig targets IL-33 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen IL-33 fusion protein Ag21430
Full Name interleukin 33
Calculated molecular weight 30 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC047085
Gene symbol IL33
Gene ID (NCBI) 90865
Conjugate Unconjugated
Form Liquid
Purification Method Thiophilic affinity chromatograph
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IL-33 is an IL-1 family cytokine and nuclear alarmin, is constitutively expressed in epithelial barrier tissues and human blood vessels. IL-33 is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. It also acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury.

Protocols

Product Specific Protocols
WB protocol for IL-33 antibody 66235-1-IgDownload protocol
IHC protocol for IL-33 antibody 66235-1-IgDownload protocol
IF protocol for IL-33 antibody 66235-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Cells

Helicobacter pylori Induces IL-33 Production and Recruits ST-2 to Lipid Rafts to Exacerbate Inflammation.

Authors - Chia-Jung Kuo
humanWB,IHC

Sci Rep

Dysfunctional EAT thickness may promote maladaptive heart remodeling in CVD patients through the ST2-IL33 system, directly related to EPAC protein expression.

Authors - Elena Vianello