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IL33 Antibody 2 Publications

Mouse Monoclonal| Catalog number: 66235-1-Ig |CloneNo.: 2A9C7

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Con: 204 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
Application not tested? Please see our Guarantee
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.


Images See all

IL33 Antibody WB human lung tissue 66235-1-Ig

human lung tissue were subjected to SDS PAGE followed by western blot with 66235-1-Ig( IL33 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

IL33 Antibody IHC human nasopharyngeal carcinoma tissue 66235-1-Ig

Immunohistochemical analysis of paraffin-embedded human nasopharyngeal carcinoma tissue slide using 66235-1-Ig( IL33 Antibody) at dilution of 1:200 (under 10x lens).

IL33 Antibody IHC human nasopharyngeal carcinoma tissue 66235-1-Ig

Immunohistochemical analysis of paraffin-embedded human nasopharyngeal carcinoma tissue slide using 66235-1-Ig( IL33 Antibody) at dilution of 1:200 (under 40x lens).

IL33 Antibody IF HepG2 cells 66235-1-Ig

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HepG2 cells using 66235-1-Ig(IL33 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:
human
Positive WB detected in:
human lung tissue, HepG2 cells
Positive IHC detected in:
human nasopharyngeal carcinoma tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:50-1:500
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Mouse
Purification method:
Thiophilic affinity chromatograph
Isotype:
IgM
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
IL33 fusion protein Ag21430
Full name:
interleukin 33
Calculated molecular weight:
30 kDa
Observed molecular weight:
35 kDa
GenBank accession number:
BC047085
Gene ID (NCBI):
90865
Gene symbol
IL33
Synonyms
C9orf26, DVS27, IL 1F11, IL 33, IL1F11, IL33, Interleukin 1 family member 11, interleukin 33, NF HEV, NFEHEV, NFHEV, RP11 575C20.2

Background

IL-33 is an IL-1 family cytokine and nuclear alarmin, is constitutively expressed in epithelial barrier tissues and human blood vessels. IL-33 is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. It also acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury.


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