IL-33 Monoclonal antibody, PBS Only

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  WB analysis of human lung using 66235-1-Ig (same clone as 66235-1-PBS)

  human lung tissue were subjected to SDS PAGE followed by western blot with 66235-1-Ig (IL33 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66235-1-PBS in a different storage buffer formulation.

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  WB analysis of THP-1 using 66235-1-Ig (same clone as 66235-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66235-1-Ig (IL-33 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66235-1-PBS in a different storage buffer formulation.

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  WB analysis of hTERT-RPE1 using 66235-1-Ig (same clone as 66235-1-PBS)

  hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 66235-1-Ig (IL-33 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66235-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66235-1-Ig (same clone as 66235-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66235-1-Ig (IL-33 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66235-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 66235-1-Ig (same clone as 66235-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66235-1-Ig (IL-33 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66235-1-PBS in a different storage buffer formulation.

IL-33 Monoclonal Antibody for WB, ELISA

Cat No. 66235-1-PBS

Clone No.2A9C7

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB, ELISA

Synonyms

C9orf26, DKFZp586H0523, DVS27, IL 1F11, IL 33, IL1F11, IL33, IL-33, Interleukin 1 family member 11, interleukin 33, NF HEV, NFEHEV, NFHEV, RP11 575C20.2

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Product Information

66235-1-PBS targets IL-33 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21430

  Product name: Recombinant human IL-33 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1-270 aa of BC047085

  Sequence: MKPKMKYSTNKISTAKWKNTASKALCFKLGKSQQKAKEVCPMYFMKLRSGLMIKKEACYFRRETTKRPSLKTGRKHKRHLVLAACQQQSTVECFAFGISGVQKYTRALHDSSITGISPITEYLASLSTYNDQSITFALEDESYEIYVEDLKKDEKKDKVLLSYYESQHPSNESGDGVDGKMLMVTLSPTKDFWLHANNKEHSVELHKCEKPLPDQAFFVLHNMHSNCVSFECKTDPGVFIGVKDNHLALIKVDSSENLCTENILFKLSET

• Full Name: interleukin 33
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC047085
• Gene Symbol: IL-33
• Gene ID (NCBI): 90865
• RRID: AB_2881624
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Thiophilic affinity chromatograph
• UNIPROT ID: O95760
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

IL-33 is an IL-1 family cytokine and nuclear alarmin, and is constitutively expressed in epithelial barrier tissues and human blood vessels. IL-33 is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. It also acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury.