|Positive WB detected in||A549 cells, rat heart tissue, HeLa cells, HepG2 cells, K-562 cells|
|Positive IP detected in||K-562 cells|
|Positive IHC detected in||human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12948-1-AP targets IMPDH2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IMPDH2 fusion protein Ag3632|
|Full Name||IMP (inosine monophosphate) dehydrogenase 2|
|Calculated molecular weight||514 aa, 56 kDa|
|Observed molecular weight||56 kDa|
|GenBank accession number||BC012840|
|Gene ID (NCBI)||3615|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
IMPDH2, also named as IMPD2, belongs to the IMPDH/GMPR family. It is a rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors. IMPDH2 is a key enzyme in the purine nucleotide biosynthetic pathway and constitutes a pivotal biological target for immunosuppressant and antiviral drugs. (PMID:21181270) IMPDH2 is highly enriched in RR(filamentous rods and rings). This antibody may has cross reaction with IMPDH1 due to the high homology.
|Product Specific Protocols|
|WB protocol for IMPDH2 antibody 12948-1-AP||Download protocol|
|IHC protocol for IMPDH2 antibody 12948-1-AP||Download protocol|
|IF protocol for IMPDH2 antibody 12948-1-AP||Download protocol|
|IP protocol for IMPDH2 antibody 12948-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Immune Response-Dependent Assembly of IMP Dehydrogenase Filaments.
Inter-filament interaction between IMPDH and CTPS cytoophidia.
Int J Colorectal Dis
Identification of IMPDH2 as a tumor-associated antigen in colorectal cancer using immunoproteomics analysis.
J Histochem Cytochem
Ultrastructure of Cytoplasmic and Nuclear Inosine-5'-Monophosphate Dehydrogenase 2 "Rods and Rings" Inclusions.