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- KD/KO Validated
IMPDH2 Polyclonal antibody
IMPDH2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 12948-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, rat heart tissue, HeLa cells, HepG2 cells, K-562 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:100-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 11 publications below |
| IHC | See 2 publications below |
| IF | See 14 publications below |
| ELISA | See 1 publications below |
Product Information
12948-1-AP targets IMPDH2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IMPDH2 fusion protein Ag3632 |
| Full Name | IMP (inosine monophosphate) dehydrogenase 2 |
| Calculated molecular weight | 514 aa, 56 kDa |
| Observed molecular weight | 56 kDa |
| GenBank accession number | BC012840 |
| Gene symbol | IMPDH2 |
| Gene ID (NCBI) | 3615 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IMPDH2, also named as IMPD2, belongs to the IMPDH/GMPR family. It is a rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors. IMPDH2 is a key enzyme in the purine nucleotide biosynthetic pathway and constitutes a pivotal biological target for immunosuppressant and antiviral drugs. (PMID:21181270) IMPDH2 is highly enriched in RR(filamentous rods and rings). This antibody may has cross reaction with IMPDH1 due to the high homology.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IMPDH2 antibody 12948-1-AP | Download protocol |
| IHC protocol for IMPDH2 antibody 12948-1-AP | Download protocol |
| IF protocol for IMPDH2 antibody 12948-1-AP | Download protocol |
| IP protocol for IMPDH2 antibody 12948-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Colorectal Dis Identification of IMPDH2 as a tumor-associated antigen in colorectal cancer using immunoproteomics analysis. | ||
J Histochem Cytochem Ultrastructure of Cytoplasmic and Nuclear Inosine-5'-Monophosphate Dehydrogenase 2 "Rods and Rings" Inclusions. |
