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IMPDH2 Antibody 17 Publications

Rabbit Polyclonal| Catalog number: 12948-1-AP

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Con: 39 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

IMPDH2 Antibody WB A549 cells 12948-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 12948-1-AP (IMPDH2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours

IMPDH2 Antibody IP K-562 cells 12948-1-AP

IP Result of anti-IMPDH2 (IP:12948-1-AP, 3ug; Detection:12948-1-AP 1:800) with K-562 cells lysate 4000ug.

IMPDH2 Antibody IHC human lung cancer tissue 12948-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 12948-1-AP( IMPDH2 Antibody) at dilution of 1:50

IMPDH2 Antibody IHC human lung cancer tissue 12948-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 12948-1-AP( IMPDH2 Antibody) at dilution of 1:50

IMPDH2 Antibody IF HeLa cells 12948-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using 12948-1-AP(IMPDH2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
A549 cells, human brain tissue, human heart tissue, K-562 cells, mouse lung tissue, rat heart tissue, Hela cells, HepG2 cells
Positive IP detected in:
K-562 cells
Positive IHC detected in:
human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:1000-1:6000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:100-1:500
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
IMPDH2 fusion protein Ag3632
Full name:
IMP (inosine monophosphate) dehydrogenase 2
Calculated molecular weight:
514aa,56 kDa
Observed molecular weight:
56 kDa
GenBank accession number:
BC012840
Gene ID (NCBI):
3615
Gene symbol
IMPDH2
Synonyms
IMP dehydrogenase 2, IMPD 2, IMPD2, IMPDH 2, IMPDH II, IMPDH2

Background

IMPDH2, also named as IMPD2, belongs to the IMPDH/GMPR family. It is a rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors. IMPDH2 is a key enzyme in the purine nucleotide biosynthetic pathway and constitutes a pivotal biological target for immunosuppressant and antiviral drugs. (PMID:21181270) IMPDH2 is highly enriched in RR(filamentous rods and rings). This antibody may has cross reaction with IMPDH1 due to the high homology.


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