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Inhibin Beta A Polyclonal antibody

Inhibin Beta A Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 22689-1-AP

Synonyms

Activin beta A chain, EDF, FRP, INHBA, Inhibin beta A chain, Inhibin β-A, inhibin, beta A

Validation Data Gallery

  • WB analysis of HeLa using 22689-1-AP

    WB analysis of HeLa using 22689-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22689-1-AP (Inhibin beta A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 22689-1-AP

    IHC staining of human lung cancer using 22689-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 22689-1-AP (Inhibin beta A antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human lung cancer using 22689-1-AP

    IHC staining of human lung cancer using 22689-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 22689-1-AP (Inhibin beta A antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHeLa cells, A549 cells, BGC-823 cells, HepG2 cells, mouse liver tissue, SGC-7901 cells
Positive IHC detected inhuman lung cancer tissue, human stomach cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 22689-1-AP is Inhibin Beta A Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Inhibin Beta A fusion protein Ag16700
Full Name inhibin, beta A
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC007858
Gene symbol INHBA
Gene ID (NCBI) 3624
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Inhibin βA (INHBA) is a ligand in the transforming growth factor β (TGF-β) superfamily. Inhibin βA forms a disulfide-linked homo-dimer known as activin A, which has been implicated in multiple biologic processes, including neoplastic progression. The inhibin βA can also bind the βB and α isoforms to form activin AB and inhibin, respectively.

Protocols

Product Specific Protocols
WB protocol for Inhibin Beta A antibody 22689-1-APDownload protocol
IHC protocol for Inhibin Beta A antibody 22689-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Biol Reprod

Deletion of Spata2 by CRISPR/Cas9n causes increased inhibin alpha expression and attenuated fertility in male mice.

Authors - Jie Zhao
View Article