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Inhibin beta A-Specific Monoclonal antibody

Inhibin beta A-Specific Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1F8D8

Cat no : 60352-1-Ig

Synonyms

Activin beta A chain, EDF, FRP, INHBA, INHBA-Specific, Inhibin beta A chain, inhibin, beta A

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using Inhibin beta A-Specific Monoclonal antibody (60352-1-Ig)

    WB analysis of HepG2 using 60352-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig liver tissue using Inhibin beta A-Specific Monoclonal antibody (60352-1-Ig)

    WB analysis of pig liver using 60352-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using Inhibin beta A-Specific Monoclonal antibody (60352-1-Ig)

    WB analysis of human testis using 60352-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using Inhibin beta A-Specific Monoclonal antibody (60352-1-Ig)

    WB analysis of L02 using 60352-1-Ig

    L02 cells were subjected to SDS PAGE followed by western blot with 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:650 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Inhibin beta A-Specific Monoclonal antibody (60352-1-Ig)

    IHC staining of human lung cancer using 60352-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Inhibin beta A-Specific Monoclonal antibody (60352-1-Ig)

    IHC staining of human lung cancer using 60352-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:150 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Inhibin beta A-Specific Monoclonal antibody (60352-1-Ig)

    IHC staining of human oesophagus cancer using 60352-1-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:150 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Inhibin beta A-Specific Monoclonal antibody (60352-1-Ig)

    IHC staining of human oesophagus cancer using 60352-1-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:150 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Inhibin beta A-Specific Monoclonal antibody (60352-1-Ig)

    IHC staining of human stomach cancer using 60352-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:150 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Inhibin beta A-Specific Monoclonal antibody (60352-1-Ig)

    IHC staining of human stomach cancer using 60352-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:150 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHepG2 cells, human testis tissue, L02 cells, pig liver tissue
Positive IHC detected inhuman lung cancer tissue, human oesophagus cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60352-1-Ig targets Inhibin beta A-Specific in WB, IHC,ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name inhibin, beta A
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberNM_002192
Gene Symbol INHBA
Gene ID (NCBI) 3624
RRIDAB_2881461
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Inhibin βA(INHBA) is a ligand in the transforming growth factor β (TGF-β) superfamily. Inhibin βA forms a disulfide-linked homo-dimer known as activin A, which has been implicated in multiple biologic processes, including neoplastic progression. The inhibin βA can also bind the βB and α isoforms to form activin AB and inhibin, respectively.

Protocols

Product Specific Protocols
WB protocol for Inhibin beta A-Specific antibody 60352-1-IgDownload protocol
IHC protocol for Inhibin beta A-Specific antibody 60352-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols