Tested Applications
| Positive WB detected in | mouse liver tissue |
| Positive IP detected in | A549 cells, mouse liver tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29976-1-AP targets IREB2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag32233 Product name: Recombinant human IREB2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 50-255 aa of BC017880 Sequence: VRNCDGFLMKKEDVMNILDWKTKQSNVEVPFFPARVLLQDFTGIPAMVDFAAMREAVKTLGGDPEKVHPACPTDLTVDHSLQIDFSKCAIQNAPNPGGGDLQKAGKLSPLKVQPKKLPCRGQTTCRGSCDSGELGRNSGTFSSQIENTPILCPFHLQPVPEPETVLKNQEVEFGRNRERLQFFKWSSRVFKNVAVIPPGTGMAHQI Predict reactive species |
| Full Name | iron-responsive element binding protein 2 |
| Calculated Molecular Weight | 105 kDa |
| Observed Molecular Weight | 100-105 kDa |
| GenBank Accession Number | BC017880 |
| Gene Symbol | IREB2 |
| Gene ID (NCBI) | 3658 |
| RRID | AB_3086201 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P48200 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IREB2 is an RNA-binding protein that acts to regulate iron levels in the cells by regulating the translation and stability of mRNAs that affect iron homeostasis under conditions when iron is depleted. When iron levels are low, this protein binds to iron-responsive elements (IRES), stem-loop structures located either in the 5' or 3' UTRs. Binding to the 5' UTR represses translation, while binding to the 3' UTR inhibits mRNA degradation. When iron is found in the cell, this protein is degraded in a F-box and leucine rich repeat protein 5-dependent manner.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for IREB2 antibody 29976-1-AP | Download protocol |
| IHC protocol for IREB2 antibody 29976-1-AP | Download protocol |
| IP protocol for IREB2 antibody 29976-1-AP | Download protocol |
| WB protocol for IREB2 antibody 29976-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IREB2 Polyclonal antibody (Cat# 29976-1-AP) has 11 citations published in journals including Blood, Nature Cell Biology, Redox Biology, NPJ Science of Food, Free Radical Biology and Medicine, International Immunopharmacology, Frontiers in Cell and Developmental Biology, Cell Signaling, Journal of Cell Science, Toxicology and Applied Pharmacology, and Neurological Research. The associated research fields center on ferroptosis, iron metabolism, ferritinophagy, and ischemia-reperfusion injury in contexts such as multiple myeloma, diabetic nephropathy, hepatotoxicity, and pulmonary fibrosis.
| Species | Application | Title |
|---|---|---|
Blood Targeting STK17B kinase activates ferroptosis and suppresses drug resistance in multiple myeloma.
| ||
Nat Cell Biol Peritumoural adipose tissue promotes ferroptosis resistance by 3-hydroxykynurenine-mediated suppression of ferritinophagy. | ||
Redox Biol Liraglutide and GLP-1(9-37) alleviated hepatic ischemia-reperfusion injury by inhibiting ferroptosis via GSK3β/Nrf2 pathway and SMAD159/Hepcidin/FTH pathway | ||
NPJ Sci Food Time-of-day difference in iron supplementation in iron-deficient pregnant model mice. | ||
Free Radic Biol Med HERC2 as a bidirectional iron metabolism switch: Synergistic NCOA4 stabilization and FBXL5 degradation drive ferroptosis-cascaded hepatotoxicity in Nd(2)O(3) nanoparticles exposure. | ||
Int Immunopharmacol Inhibiting ubiquitin-specific protease 38 safeguards against diabetic nephropathy by limiting tubular epithelial cell ferroptotic death through the suppression of IREB2-mediated iron overload. |
