IREB2 Polyclonal antibody

IREB2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 23829-1-AP

Synonyms

ACO3, IRE BP 2, IREB2, Iron regulatory protein 2, IRP2, IRP2AD



Tested Applications

Positive WB detected inmouse lung tissue, A549 cells, PC-13 cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 23829-1-AP is IREB2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IREB2 fusion protein Ag20653
Full Name iron-responsive element binding protein 2
Calculated molecular weight 105 kDa
Observed molecular weight 90-105 kDa
GenBank accession numberBC017880
Gene symbol IREB2
Gene ID (NCBI) 3658
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IREB2 antibody 23829-1-APDownload protocol
IHC protocol for IREB2 antibody 23829-1-APDownload protocol
IF protocol for IREB2 antibody 23829-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nutrients

Iron Transport from Ferrous Bisglycinate and Ferrous Sulfate in DMT1-Knockout Human Intestinal Caco-2 Cells.

Authors - Xiaonan Yu
humanWB

Cancer Manag Res

MiR-935 Promotes Clear Cell Renal Cell Carcinoma Migration and Invasion by Targeting IREB2.

Authors - Fei Liu
humanWB,IF,CoIP

Free Radic Biol Med

Iron regulatory protein 2 is required for artemether -mediated anti-hepatic fibrosis through ferroptosis pathway.

Authors - Yujia Li
  • KD Validated