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  • KD/KO Validated

IREB2 Polyclonal antibody

IREB2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 23829-1-AP

Synonyms

ACO3, IRE BP 2, IREB2, Iron regulatory protein 2, IRP2, IRP2AD



Tested Applications

Positive WB detected inmouse lung tissue, A549 cells, PC-13 cells
Positive IHC detected inhuman spleen tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23829-1-AP targets IREB2 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IREB2 fusion protein Ag20653
Full Name iron-responsive element binding protein 2
Calculated Molecular Weight 105 kDa
Observed Molecular Weight 90-105 kDa
GenBank Accession NumberBC017880
Gene Symbol IREB2
Gene ID (NCBI) 3658
RRIDAB_2879331
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IREB2 is an RNA-binding protein that acts to regulate iron levels in the cells by regulating the translation and stability of mRNAs that affect iron homeostasis under conditions when iron is depleted. When iron levels are low, this protein binds to iron-responsive elements (IRES), stem-loop structures located either in the 5' or 3' UTRs. Binding to the 5' UTR represses translation, while binding to the 3' UTR inhibits mRNA degradation. When iron is found in the cell, this protein is degraded in a F-box and leucine rich repeat protein 5-dependent manner.

Protocols

Product Specific Protocols
WB protocol for IREB2 antibody 23829-1-APDownload protocol
IHC protocol for IREB2 antibody 23829-1-APDownload protocol
IF protocol for IREB2 antibody 23829-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

EMBO Rep

The deubiquitinase OTUD1 enhances iron transport and potentiates host antitumor immunity.

Authors - Jia Song
humanWB

Cell Mol Life Sci

Phenazine derivatives attenuate the stemness of breast cancer cells through triggering ferroptosis.

Authors - Yue Yang
humanWB,IF,CoIP

Free Radic Biol Med

Iron regulatory protein 2 is required for artemether -mediated anti-hepatic fibrosis through ferroptosis pathway.

Authors - Yujia Li
  • KD Validated
mouseWB

Biochim Biophys Acta Mol Basis Dis

Iron deposition-induced ferroptosis in alveolar type II cells promotes the development of pulmonary fibrosis.

Authors - Haipeng Cheng
humanWB

Nutrients

Iron Transport from Ferrous Bisglycinate and Ferrous Sulfate in DMT1-Knockout Human Intestinal Caco-2 Cells.

Authors - Xiaonan Yu
mouseWB,IF

Neurochem Int

Activation of vitamin D receptor inhibits Tau phosphorylation is associated with reduction of iron accumulation in APP/PS1 transgenic mice.

Authors - Ting-Yao Wu