- Featured Product
- KD/KO Validated
IRF1 Polyclonal antibody
IRF1 Polyclonal Antibody for WB, IF, FC, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IF, FC, IHC, CoIP, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 11335-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | IFN gamma treated HeLa cells, HL-60 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | IFN gamma treated HepG2 cells |
| Positive FC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 26 publications below |
| IHC | See 5 publications below |
| IF | See 6 publications below |
| CoIP | See 1 publications below |
| ChIP | See 1 publications below |
Product Information
11335-1-AP targets IRF1 in WB, IF, FC, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IRF1 fusion protein Ag1879 |
| Full Name | IRF 1 |
| Calculated Molecular Weight | 325 aa, 37 kDa |
| Observed Molecular Weight | 45-50 kDa |
| GenBank Accession Number | BC009483 |
| Gene Symbol | IRF1 |
| Gene ID (NCBI) | 3659 |
| RRID | AB_2877759 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IRF1, also named as IFN regulatory factor 1, is a 325 amino acid protein, which belongs to the IRF family. IRF1 as a transcriptional regulator which displays a remarkable functional diversity in the regulation of cellular responses. The calculated molecular weight of IRF1 is 37 kDa, but the modified IRF1 protein is about 45-50 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IRF1 antibody 11335-1-AP | Download protocol |
| IHC protocol for IRF1 antibody 11335-1-AP | Download protocol |
| IF protocol for IRF1 antibody 11335-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Immunother Cancer Nanoparticle delivery of miR-21-3p sensitizes melanoma to anti-PD-1 immunotherapy by promoting ferroptosis. | ||
Free Radic Biol Med IRF1/ZNF350/GPX4-mediated ferroptosis of renal tubular epithelial cells promote chronic renal allograft interstitial fibrosis
| ||
Front Immunol Toxoplasma Effector TgIST Targets Host IDO1 to Antagonize the IFN-γ-Induced Anti-parasitic Response in Human Cells. | ||
Cancer Commun (Lond) MAGE-C3 promotes cancer metastasis by inducing epithelial-mesenchymal transition and immunosuppression in esophageal squamous cell carcinoma. | ||
Int J Mol Sci Comprehensive Analysis of PANoptosis-Related Gene Signature of Ulcerative Colitis | ||
Inflammation Chlorogenic Acid Alleviates Hepatic Ischemia-Reperfusion Injury by Inhibiting Oxidative Stress, Inflammation, and Mitochondria-Mediated Apoptosis In Vivo and In Vitro |
