Integrin beta-1 Antibody

Integrin beta-1 Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

FC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A7C9

Cat no : 66315-1-Ig

Synonyms

CD29, FNRB, GPIIA, Integrin beta 1, Integrin beta-1, ITGB1, MDF2, MSK12, VLA 4 subunit beta, VLA BETA, VLAB



Tested Applications

Positive WB detected inHT-1080 cells, HUVEC cells
Positive FC detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Sample-dependent, check data in validation data gallery

Published Applications

FCSee 1 publications below

Product Information

66315-1-Ig targets Integrin beta-1 in FC, WB, ELISA applications and shows reactivity with human samples.

Reactivity human
Cited Species rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Integrin beta-1 fusion protein Ag24476
Full Name integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
Calculated molecular weight 88 kDa
Observed molecular weight 130 kDa
GenBank accession number BC020057
Gene symbol ITGB1
Gene ID (NCBI) 3688
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

Integrin beta-1 (ITGB1), also named as CD29, is a 130 kDa single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.

Protocols

Product Specific Protocols
WB protocol for Integrin beta-1 antibody 66315-1-IgDownload protocol
FC protocol for Integrin beta-1 antibody 66315-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratFC

J Cell Mol Med

Osthole stimulates bone formation, drives vascularization and retards adipogenesis to alleviate alcohol-induced osteonecrosis of the femoral head.

Authors - Hongping Yu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ryan MacDonald (01-24-2018)

Antigen retrieval - Tris HCl 0.1M ph 9

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Zebrafish Retina/Brain
Integrin beta-1 Antibody Immunohistochemistry, validation (1:100 dilution) in Zebrafish Retina/Brain (Cat no:66315-1-Ig)