ATG13 Monoclonal antibody

ATG13 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1C3A7

Cat no : 66708-1-Ig

Synonyms

ATG13, Autophagy related protein 13, FLJ20698, KIAA0652



Tested Applications

Positive WB detected inmouse brain tissue, BGC-823 cells, NIH/3T3 cells, pig brain tissue, rat brain tissue
Positive IHC detected inmouse testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66708-1-Ig targets ATG13 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATG13 fusion protein Ag12968
Full Name KIAA0652
Calculated molecular weight 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC001331
Gene symbol KIAA0652
Gene ID (NCBI) 9776
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATG13 is one component protein of the ULK1 complex which is required for autophagosome formation and mitophagy. ATG13 has two nutrient regulatory phosphorylation sites and the phosphorylation status of ATG13 affect regulation of autophagy by modulating enzyme activity and cellular localization of ULK1. Besides, it has been reported the nonautophagic function of ATG13 on cardiac development for ATG13-deficient embryos show myocardial growth defects.(PMID:27387056, 26801615, 26644405)

Protocols

Product Specific Protocols
WB protocol for ATG13 antibody 66708-1-IgDownload protocol
IHC protocol for ATG13 antibody 66708-1-IgDownload protocol
IF protocol for ATG13 antibody 66708-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols