|Positive WB detected in||NIH/3T3 cells, human placenta tissue, mouse brain tissue|
|Positive IHC detected in||human liver cancer tissue, human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||NIH/3T3 cells|
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 18375-1-AP is KISS1 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KISS1 fusion protein Ag13240|
|Full Name||KiSS-1 metastasis-suppressor|
|Calculated molecular weight||15 kDa|
|Observed molecular weight||16-20 kDa|
|GenBank accession number||BC022819|
|Gene ID (NCBI)||3814|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
KISS1, also known as kisspeptin, is a metastasis suppressor protein that suppresses metastases of human melanomas and breast carcinomas without affecting tumorigenicity. It may regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. A C-terminally amidated peptide of KISS1, metastin (kisspeptin-54 or kp-54), functions as the endogenous ligand of the G-protein coupled receptor GPR54 (PMID: 11457843; 11385580). KISS1-GPR54 signaling is involved in the regulation of reproduction, playing an important role in initiating secretion of gonadotropin-releasing hormone (GnRH) at puberty (PMID: 18753370).
Analysis of the association of the expression of KiSS-1 in colorectal cancer tissues with the pathology and prognosis.
Histochem Cell Biol
Functional analysis of miR-767-5p during the progression of hepatocellular carcinoma and the clinical relevance of its dysregulation.
Int J Cancer
Long noncoding RNA TC0101441 induces epithelial-mesenchymal transition in epithelial ovarian cancer metastasis by downregulating KiSS1.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Kristina (Verified Customer) (09-18-2020)
Works great for us- seeing an increase in modified MDA231 cells.