Cytokeratin 19 Polyclonal antibody

Cytokeratin 19 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10712-1-AP

Synonyms

CK 19, CK19, Cytokeratin 19, K19, K1CS, keratin 19, KRT19



Tested Applications

Positive WB detected inA431 cells, A549 cells, HepG2 cells, MCF-7 cells, mouse brain tissue, mouse placenta tissue
Positive IHC detected inhuman colon tissue, mouse skin tissue, mouse colon tissue, human stomach cancer tissue, human lung cancer tissue, human skin tissue, human liver cancer tissue, rat liver tissue, human liver tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:1000-1:6400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10712-1-AP targets Cytokeratin 19 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 19 fusion protein Ag1085
Full Name keratin 19
Calculated molecular weight 40 kDa
Observed molecular weight 44-50 kDa
GenBank accession numberBC007628
Gene symbol KRT19
Gene ID (NCBI) 3880
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 19 is a type I cytokeratin. It is a biochemical marker of skin stem cells in vivo and in vitro.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 19 antibody 10712-1-APDownload protocol
IHC protocol for Cytokeratin 19 antibody 10712-1-APDownload protocol
IF protocol for Cytokeratin 19 antibody 10712-1-APDownload protocol
FC protocol for Cytokeratin 19 antibody 10712-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Oncol Lett

Cytokeratin expression in epidermal stem cells in skin adnexal tumors.

Authors - Xiaoqiu Zhou
humanIHC

Oncogene

THZ1 reveals CDK7-dependent transcriptional addictions in pancreatic cancer.

Authors - Ping Lu
IF

Dis Model Mech

A novel pancreatic cancer model originated from transformation of acinar cells in adult tree shrew, a primate-like animal.

Authors - Qiu Tu
humanIF

Vaccines (Basel)

Neutralizing Monoclonal Antibodies Reduce Human Cytomegalovirus Infection and Spread in Developing Placentas.

Authors - Takako Tabata
ratIF

Med Sci Monit

PMID: 31929497

Authors - Fang Xu
mouseIHC,IF

Cell Death Dis

Myofibroblast induces hepatocyte-to-ductal metaplasia via laminin-ɑvβ6 integrin in liver fibrosis.

Authors - Ting Xu