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  • Featured Product
  • KD/KO Validated

Cytokeratin 6B Polyclonal antibody

Cytokeratin 6B Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 17391-1-AP

Synonyms

CK 6B, CK6B, Cytokeratin 6B, K6B, keratin 6B, KRT6B, KRTL1, Type II keratin Kb10

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skin tissue using Cytokeratin 6B Polyclonal antibody (17391-1-AP)

    WB analysis of mouse skin using 17391-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using Cytokeratin 6B Polyclonal antibody (17391-1-AP)

    WB analysis of A431 using 17391-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat skin tissue using Cytokeratin 6B Polyclonal antibody (17391-1-AP)

    WB analysis of rat skin using 17391-1-AP

    rat skin tissue were subjected to SDS PAGE followed by western blot with 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Cytokeratin 6B Polyclonal antibody (17391-1-AP)

    IHC staining of human lung cancer using 17391-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17391-1-AP (Cytokeratin 6B antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Cytokeratin 6B Polyclonal antibody (17391-1-AP)

    IHC staining of human lung cancer using 17391-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17391-1-AP (Cytokeratin 6B antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 6B Polyclonal antibody (17391-1-AP)

    IHC staining of human cervical cancer using 17391-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cytokeratin 6B Polyclonal antibody (17391-1-AP)

    IHC staining of human cervical cancer using 17391-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cytokeratin 6B Polyclonal antibody (17391-1-AP)

    IHC staining of human breast cancer using 17391-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsil tissue using Cytokeratin 6B Polyclonal antibody (17391-1-AP)

    IHC staining of human tonsil using 17391-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsil using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human tonsil tissue using Cytokeratin 6B Polyclonal antibody (17391-1-AP)

    IHC staining of human tonsil using 17391-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsil using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human oesophagus tissue using Cytokeratin 6B Polyclonal antibody (17391-1-AP)

    IHC staining of human oesophagus using 17391-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human oesophagus tissue using Cytokeratin 6B Polyclonal antibody (17391-1-AP)

    IHC staining of human oesophagus using 17391-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cytokeratin 6B Polyclonal antibody (17391-1-AP)

    IHC staining of human breast cancer using 17391-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cytokeratin 6B Polyclonal antibody (17391-1-AP)

    IF Staining of HeLa using 17391-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 6B antibody (17391-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using Cytokeratin 6B Polyclonal antibody (17391-1-AP)

    FC experiment of HeLa using 17391-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 6B (17391-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inmouse skin tissue, A431 cells, rat skin tissue
Positive IHC detected inhuman lung cancer tissue, human cervical cancer tissue, human breast cancer tissue, human tonsil tissue, human oesophagus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1600-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17391-1-AP targets Cytokeratin 6B in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 6B fusion protein Ag11288
Full Name keratin 6B
Calculated Molecular Weight 564 aa, 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC034535
Gene Symbol KRT6B
Gene ID (NCBI) 3854
RRIDAB_2134318
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 6 is a type II keratin. It is used as a marker for epidermal hyperproliferation and differentiation. Three keratin 6 isoforms were found, ketatin 6A,6B, and 6C. They share more than 99% identical DNA sequence.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 6B antibody 17391-1-APDownload protocol
IHC protocol for Cytokeratin 6B antibody 17391-1-APDownload protocol
IF protocol for Cytokeratin 6B antibody 17391-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

J Invest Dermatol

Frizzled6 deficiency disrupts the differentiation process of nail development.

Authors - Cui Chang-Yi CY
View Article
humanWB

J Transl Med

Bladder cancer-derived exosomal KRT6B promotes invasion and metastasis by inducing EMT and regulating the immune microenvironment.

Authors - Qiang Song
  • KD Validated
View Article