Cytokeratin 8 Polyclonal antibody

Cytokeratin 8 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 17514-1-AP

Synonyms

CARD2, CK 8, CK8, CYK8, Cytokeratin 8, K2C8, K8, keratin 8, KO, KRT8, Type II keratin Kb8



Tested Applications

Positive WB detected inMCF-7 cells, A431 cells, A549 cells, C6 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue, human colon tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

17514-1-AP targets Cytokeratin 8 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivity hamster, human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name keratin 8
Calculated molecular weight 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberNM_002273
Gene symbol KRT8
Gene ID (NCBI) 3856
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo. This antibody is specifically against KRT8.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 8 antibody 17514-1-APDownload protocol
IHC protocol for Cytokeratin 8 antibody 17514-1-APDownload protocol
IF protocol for Cytokeratin 8 antibody 17514-1-APDownload protocol
FC protocol for Cytokeratin 8 antibody 17514-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Exp Med

Wnt5a induces and maintains prostate cancer cells dormancy in bone.

Authors - Dong Ren
humanIHC

Oncol Lett

Cytokeratin expression in epidermal stem cells in skin adnexal tumors.

Authors - Xiaoqiu Zhou
mouseIHC

J Cancer Res Clin Oncol

Animal and cellular models of hepatocellular carcinoma bone metastasis: establishment and characterisation.

Authors - Rui Hou
human, hamsterWB

Cancer Gene Ther

Evaluation of apoptogenic adenovirus type 5 oncolytic vectors in a Syrian hamster head and neck cancer model.

Authors - S Vijayalingam
mouseIHC

Sci Rep

Conversion of embryonic stem cells into extraembryonic lineages by CRISPR-mediated activators.

Authors - Shu Wei

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Joleen (Verified Customer) (06-12-2019)

This antibody recognizes a band that matched with the expected molecular weight of KRT8.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
Cytokeratin 8 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:17514-1-AP)