Tested Applications
| Positive WB detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83415-3-RR targets Cytokeratin 77 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag21942 Product name: Recombinant human KRT77 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 490-578 aa of BC122558 Sequence: QNSQVSVNGGAGGGGSYGSGGYGGGSGGGYGGGRSYRGGGARGGSGGGYGSGCGGGGGSYGGSGRSGRGSSRVQIIQTSTNTSHRRILE Predict reactive species |
| Full Name | keratin 77 |
| Calculated Molecular Weight | 578 aa, 62 kDa |
| Observed Molecular Weight | 61 kDa |
| GenBank Accession Number | BC122558 |
| Gene Symbol | KRT77 |
| Gene ID (NCBI) | 374454 |
| RRID | AB_3671064 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q7Z794 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins.The KRT77 gene is a type II epithelial cell α-keratin gene family member that is expressed in the skin and eccrine sweat glands. KRT77 has N- and C-terminal domains and a central rod domain consisting of 4 alpha-helical regions separated by 3 nonhelical linker sequences.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Cytokeratin 77 antibody 83415-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
