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LAMC1 Polyclonal antibody

LAMC1 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP,ELISA

Conjugate

Unconjugated

Cat no : 20928-1-AP

Synonyms

LAMB2, LAMC1, Laminin 1 subunit gamma, Laminin 10 subunit gamma, Laminin 11 subunit gamma, Laminin 2 subunit gamma, Laminin 3 subunit gamma, Laminin 4 subunit gamma, Laminin 6 subunit gamma, Laminin 7 subunit gamma, Laminin 8 subunit gamma, Laminin 9 subunit gamma, Laminin B2 chain, Laminin subunit gamma 1, S LAM gamma, S laminin subunit gamma

Validation Data Gallery

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  • Western Blot (WB) analysis of DU 145 cells using LAMC1 Polyclonal antibody (20928-1-AP)

    WB analysis of DU 145 using 20928-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 20928-1-AP (LAMC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using LAMC1 Polyclonal antibody (20928-1-AP)

    WB analysis of HeLa using 20928-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20928-1-AP (LAMC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of DU 145 cells using LAMC1 Polyclonal antibody (20928-1-AP)

    IP experiment of DU 145 using 20928-1-AP

    IP result of anti-LAMC1 (IP:20928-1-AP, 4ug; Detection:20928-1-AP 1:1000) with DU 145 cells lysate 3600ug.

Tested Applications

Positive WB detected inDU 145 cells, HeLa cells, A431 cells
Positive IP detected inDU 145 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20928-1-AP is LAMC1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LAMC1 fusion protein Ag14674
Full Name laminin, gamma 1 (formerly LAMB2)
Calculated Molecular Weight 178 kDa
Observed Molecular Weight 220 kDa, 178 kDa
GenBank Accession NumberBC015586
Gene Symbol LAMC1
Gene ID (NCBI) 3915
RRIDAB_2878767
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LAMC1 antibody 20928-1-APDownload protocol
IP protocol for LAMC1 antibody 20928-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols