LARS Polyclonal antibody

LARS Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21146-1-AP

Synonyms

hr025Cl, HSPC192, KIAA1352, LARS, LARS1, Leucine tRNA ligase, leucyl tRNA synthetase, LEURS, LEUS, LRS, PIG44, RNTLS



Tested Applications

Positive WB detected inA549 cells, Y79 cells, HeLa cells, Jurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman colon tissue, human brain tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 21146-1-AP is LARS Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LARS fusion protein Ag15684
Full Name leucyl-tRNA synthetase
Calculated molecular weight 1176 aa, 134 kDa
Observed molecular weight 135-140 kDa
GenBank accession numberBC151214
Gene symbol LARS
Gene ID (NCBI) 51520
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for LARS antibody 21146-1-APDownload protocol
IHC protocol for LARS antibody 21146-1-APDownload protocol
IP protocol for LARS antibody 21146-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS One

A Genome-Wide siRNA Screen in Mammalian Cells for Regulators of S6 Phosphorylation.

Authors - Angela Papageorgiou