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LDHAL6A Polyclonal antibody

LDHAL6A Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 16221-1-AP

Synonyms

LDHAL6A, LDHL2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using LDHAL6A Polyclonal antibody (16221-1-AP)

    WB analysis of mouse liver using 16221-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16221-1-AP (LDHAL6A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using LDHAL6A Polyclonal antibody (16221-1-AP)

    WB analysis of human brain using 16221-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16221-1-AP (LDHAL6A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using LDHAL6A Polyclonal antibody (16221-1-AP)

    WB analysis of Jurkat using 16221-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16221-1-AP (LDHAL6A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using LDHAL6A Polyclonal antibody (16221-1-AP)

    WB analysis of mouse testis using 16221-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 16221-1-AP (LDHAL6A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using LDHAL6A Polyclonal antibody (16221-1-AP)

    WB analysis of mouse brain using 16221-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16221-1-AP (LDHAL6A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LDHAL6A Polyclonal antibody (16221-1-AP)

    IF Staining of HeLa using 16221-1-AP

    Immunofluorescent analysis of Hela cells, using LDHAL6A antibody 16221-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inmouse liver tissue, human brain tissue, Jurkat cells, mouse brain tissue, mouse testis tissue
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16221-1-AP targets LDHAL6A in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LDHAL6A fusion protein Ag9273
Full Name lactate dehydrogenase A-like 6A
Calculated Molecular Weight 26 kDa, 37 kDa
Observed Molecular Weight 30-36 kDa
GenBank Accession NumberBC014340
Gene Symbol LDHAL6A
Gene ID (NCBI) 160287
RRIDAB_2265629
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LDHAL6A antibody 16221-1-APDownload protocol
IF protocol for LDHAL6A antibody 16221-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols