• Featured Product
  • KD/KO Validated

LDHB-Specific Polyclonal antibody

LDHB-Specific Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 19988-1-AP

Synonyms

lactate dehydrogenase B, LDH B, LDH H, LDH heart subunit, LDHB, TRG 5



Tested Applications

Positive WB detected inmouse heart tissue, HeLa cells, human heart tissue
Positive IHC detected inhuman heart tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1500
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

19988-1-AP targets LDHB-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name lactate dehydrogenase B
Calculated molecular weight 37 kDa
Observed molecular weight 35 kDa
GenBank accession numberNM_002300
Gene symbol LDHB
Gene ID (NCBI) 3945
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LDHB, also named as NY-REN-46 and LDH-H, belongs to the LDH/MDH superfamily and LDH family. LDHB catalyzes the reaction: (S)-lactate + NAD+ = pyruvate + NADH. It is an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway. It is glycolytic enzymes and it is positively regulated by mTOR.Defects in LDHB are a cause of hereditary LDHB deficiency. The antibody is specific to LDHB and has no cross reaction with LDHA and LDHC.

Protocols

Product Specific Protocols
WB protocol for LDHB-Specific antibody 19988-1-APDownload protocol
IHC protocol for LDHB-Specific antibody 19988-1-APDownload protocol
IF protocol for LDHB-Specific antibody 19988-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols