Tested Applications
| Positive WB detected in | DU 145 cells, HeLa cells, HEK-293T cells, Jurkat cells, PC-3 cells, mouse heart tissue, rat heart tissue, mouse brain tissue, mouse brain cortex tissue, mouse cerebellum tissue |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19988-1-AP targets LDHB-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | lactate dehydrogenase B |
| Calculated Molecular Weight | 37 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | NM_002300 |
| Gene Symbol | LDHB |
| Gene ID (NCBI) | 3945 |
| RRID | AB_10638780 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P07195 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LDHB, also named as NY-REN-46 and LDH-H, belongs to the LDH/MDH superfamily and LDH family. LDHB catalyzes the reaction: (S)-lactate + NAD+ = pyruvate + NADH. It is an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway. It is glycolytic enzymes and it is positively regulated by mTOR.Defects in LDHB are a cause of hereditary LDHB deficiency. The antibody is specific to LDHB and has no cross reaction with LDHA and LDHC.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for LDHB-Specific antibody 19988-1-AP | Download protocol |
| IHC protocol for LDHB-Specific antibody 19988-1-AP | Download protocol |
| WB protocol for LDHB-Specific antibody 19988-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-LDHA (Cat# 19988-1-AP) has 10 total citations. Journals include EMBO Reports, Molecular Cell, iScience, Drug Resistance Updates, Biomedicines, Am J Physiol Renal Physiol, Mol Biol Rep, Prostate, and J Nutr Sci Vitaminol. Research fields span cancer biology (bladder, head and neck, prostate), lactate metabolism, mitophagy, neuroscience and cognition, cell cycle regulation, and post-translational modification studies.
| Species | Application | Title |
|---|---|---|
Drug Resist Updat Lactate-driven H3K18 lactylation promotes cisplatin resistance in bladder cancer via HNRNPF-Parkin mediated mitophagy. | ||
EMBO Rep CDK1-mediated phosphorylation of LDHA fuels mitosis through LDHB-dependent lactate oxidation.
| ||
iScience Lactate-driven type I collagen deposition facilitates cancer stem cell-like phenotype of head and neck squamous cell carcinoma
| ||
iScience Altered neuronal lactate dehydrogenase A expression affects cognition in a sex- and age-dependent manner | ||
Biomedicines HMTM-Mediated Enhancement of Brain Bioenergetics in a Mouse Tauopathy Model Is Blocked by Chronic Administration of Rivastigmine. | ||
Am J Physiol Renal Physiol Expression of lactate dehydrogenase A and B isoforms in the mouse kidney. |
Reviews
What customers say
The single review for 19988-1-AP is from Amy Spicer, who used the antibody for Western Blot at a 1:1000 dilution (lot 00052082). She rated it 5 out of 5 stars and commented, "Fantastic, works well." Overall, the feedback is positive, indicating reliable performance in WB applications at the tested dilution.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Amy (Verified Customer) (02-06-2026) | Fantastic, works well.
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