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LDHB Monoclonal antibody

LDHB Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

5E9G1

Cat no : 66425-1-Ig

Synonyms

lactate dehydrogenase B, LDH B, LDH H, LDH heart subunit, LDHB, TRG 5

Validation Data Gallery

  • WB analysis using 66425-1-Ig

    WB analysis using 66425-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66425-1-Ig (LDHB Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 66425-1-Ig

    IF Staining of HeLa using 66425-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66425-1-Ig (LDHB antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HEK293 cells, Jurkat cells, ROS1728 cells, C6 cells, RAW264.7 cells, NIH/3T3 cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:10000-1:50000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66425-1-Ig targets LDHB in WB, IF, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivity rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LDHB fusion protein Ag6605
Full Name lactate dehydrogenase B
Calculated molecular weight 36 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC002362
Gene symbol LDHB
Gene ID (NCBI) 3945
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LDHB, also named as LDH-H and NY-REN-46, belongs to the LDH/MDH superfamily. And LDH family. It is an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway.It is glycolytic enzymes and it is positively regulated by mTOR.

Protocols

Product Specific Protocols
WB protocol for LDHB antibody 66425-1-IgDownload protocol
IF protocol for LDHB antibody 66425-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IF

Cell Death Dis

PDK4 promotes vascular calcification by interfering with autophagic activity and metabolic reprogramming.

Authors - Wen-Qi Ma
View Article