LDHB Monoclonal antibody
LDHB Monoclonal Antibody for IF, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, rat, mouse
Applications
WB, IF, ELISA
Conjugate
Unconjugated
CloneNo.
5E9G1
Cat no : 66425-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HEK293 cells, Jurkat cells, ROS1728 cells, C6 cells, RAW264.7 cells, NIH/3T3 cells |
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:10000-1:50000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 1 publications below |
IF | See 1 publications below |
Product Information
66425-1-Ig targets LDHB in WB, IF, ELISA applications and shows reactivity with human, rat, mouse samples.
Tested Reactivity | human, rat, mouse |
Cited Reactivity | rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | LDHB fusion protein Ag6605 |
Full Name | lactate dehydrogenase B |
Calculated molecular weight | 36 kDa |
Observed molecular weight | 35 kDa |
GenBank accession number | BC002362 |
Gene symbol | LDHB |
Gene ID (NCBI) | 3945 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LDHB, also named as LDH-H and NY-REN-46, belongs to the LDH/MDH superfamily. And LDH family. It is an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway.It is glycolytic enzymes and it is positively regulated by mTOR.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for LDHB antibody 66425-1-Ig | Download protocol |
IF protocol for LDHB antibody 66425-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Death Dis PDK4 promotes vascular calcification by interfering with autophagic activity and metabolic reprogramming. |