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Tested Applications
| Positive WB detected in | mouse kidney tissue, DU 145 cells, NIH/3T3 cells |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
Product Information
55003-1-AP targets LIN41 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | tripartite motif-containing 71 |
| Calculated Molecular Weight | 93 kDa |
| Observed Molecular Weight | 93 kDa |
| GenBank Accession Number | NM_001039111 |
| Gene Symbol | LIN41 |
| Gene ID (NCBI) | 131405 |
| RRID | AB_2616313 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q2Q1W2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TRIM71, also named as LIN41, belongs to the TRIM/RBCC family. It may be involved in controlling the timing of organ formation during development. The antibody is specific to TRIM71.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LIN41 antibody 55003-1-AP | Download protocol |
| IHC protocol for LIN41 antibody 55003-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Cycle TRIM71 binds to IMP1 and is capable of positive and negative regulation of target RNAs. | ||
Cell Mol Gastroenterol Hepatol HDLBP promotes hepatocellular carcinoma proliferation and sorafenib resistance by suppressing Trim71-dependent RAF1 degradation | ||
Mol Carcinog Circ_0007429/miR-637/TRIM71/Ago2 axis participates in the regulation of proliferation, migration, invasion, apoptosis, and aerobic glycolysis of HCC |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Daniel (Verified Customer) (10-26-2022) | This antibody produces very high background, using either milk or Licor blocking buffers, PVDF or nitrocellulose.
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