LIN41 Polyclonal antibody

LIN41 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55003-1-AP

Synonyms

LIN 41, LIN41, Protein lin 41 homolog, TRIM71, tripartite motif containing 71



Tested Applications

Positive WB detected inmouse kidney tissue, DU 145 cells, NIH/3T3 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55003-1-AP targets LIN41 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name tripartite motif-containing 71
Calculated Molecular Weight 93 kDa
Observed Molecular Weight 93 kDa
GenBank Accession NumberNM_001039111
Gene Symbol TRIM71
Gene ID (NCBI) 131405
RRIDAB_2616313
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM71, also named as LIN41, belongs to the TRIM/RBCC family. It may be involved in controlling the timing of organ formation during development. The antibody is specific to TRIM71.

Protocols

Product Specific Protocols
WB protocol for LIN41 antibody 55003-1-APDownload protocol
IHC protocol for LIN41 antibody 55003-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Cycle

TRIM71 binds to IMP1 and is capable of positive and negative regulation of target RNAs.

Authors - Daniel J Foster
humanWB

Cell Mol Gastroenterol Hepatol

HDLBP promotes hepatocellular carcinoma proliferation and sorafenib resistance by suppressing Trim71-dependent RAF1 degradation

Authors - Jingsheng Yuan
human,mouseIHC

Mol Carcinog

Circ_0007429/miR-637/TRIM71/Ago2 axis participates in the regulation of proliferation, migration, invasion, apoptosis, and aerobic glycolysis of HCC

Authors - Lilan Fan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Daniel (Verified Customer) (10-26-2022)

This antibody produces very high background, using either milk or Licor blocking buffers, PVDF or nitrocellulose.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HeLa, MT4 cells
LIN41 Antibody Western Blot validation (1:500 dilution) in HeLa, MT4 cells (Cat no:55003-1-AP)