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LIN41 Polyclonal antibody

LIN41 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55003-1-AP

Synonyms

LIN 41, LIN41, Protein lin 41 homolog, TRIM71, tripartite motif containing 71

Validation Data Gallery

  • WB analysis of mouse kidney using 55003-1-AP

    WB analysis of mouse kidney using 55003-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55003-1-AP (LIN41 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 55003-1-AP

    IHC staining of human testis using 55003-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 55003-1-AP (LIN41 antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inmouse kidney tissue, DU 145 cells, NIH/3T3 cells
Positive IHC detected inhuman testis
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

55003-1-AP targets LIN41 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name tripartite motif-containing 71
Calculated molecular weight 93 kDa
Observed molecular weight 93 kDa
GenBank accession numberNM_001039111
Gene symbol TRIM71
Gene ID (NCBI) 131405
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

TRIM71, also named as LIN41, belongs to the TRIM/RBCC family. It may be involved in controlling the timing of organ formation during development. The antibody is specific to TRIM71.

Protocols

Product Specific Protocols
WB protocol for LIN41 antibody 55003-1-APDownload protocol
IHC protocol for LIN41 antibody 55003-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Cycle

TRIM71 binds to IMP1 and is capable of positive and negative regulation of target RNAs.

Authors - Daniel J Foster
View Article