Tested Applications
| Positive WB detected in | mouse kidney tissue, DU 145 cells, NIH/3T3 cells |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55003-1-AP targets LIN41 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | tripartite motif-containing 71 |
| Calculated Molecular Weight | 93 kDa |
| Observed Molecular Weight | 93 kDa |
| GenBank Accession Number | NM_001039111 |
| Gene Symbol | LIN41 |
| Gene ID (NCBI) | 131405 |
| RRID | AB_2616313 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q2Q1W2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TRIM71, also named as LIN41, belongs to the TRIM/RBCC family. It may be involved in controlling the timing of organ formation during development. The antibody is specific to TRIM71.
Publications
What published studies show
The anti-TRIM71 antibody (Cat# 55003-1-AP) has 3 total citations. These appear in Cell Mol Gastroenterol Hepatol, Cell Cycle, and Mol Carcinog. The associated research fields include hepatocellular carcinoma (HCC) proliferation and sorafenib resistance, RNA regulation via TRIM71–IMP1 interactions, and the circ_0007429/miR-637/TRIM71/Ago2 axis in cancer cell proliferation, migration, invasion, apoptosis, and aerobic glycolysis. Applications used were WB and IHC in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Cell Mol Gastroenterol Hepatol HDLBP promotes hepatocellular carcinoma proliferation and sorafenib resistance by suppressing Trim71-dependent RAF1 degradation | ||
Cell Cycle TRIM71 binds to IMP1 and is capable of positive and negative regulation of target RNAs. | ||
Mol Carcinog Circ_0007429/miR-637/TRIM71/Ago2 axis participates in the regulation of proliferation, migration, invasion, apoptosis, and aerobic glycolysis of HCC |
Reviews
What customers say
One reviewer reported using this antibody at 1:500 dilution for Western Blot on HeLa and MT4 cells. They noted that it produced very high background regardless of whether milk or Li-Cor blocking buffers were used, and whether PVDF or nitrocellulose membranes were employed. Overall, the experience was unsatisfactory due to the persistent background issue.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Daniel (Verified Customer) (10-26-2022) | This antibody produces very high background, using either milk or Licor blocking buffers, PVDF or nitrocellulose.
 |
