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Tested Applications
| Positive WB detected in | mouse brain tissue, mouse heart tissue, mouse ovary tissue |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24866-1-AP targets LRCH2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21701 Product name: Recombinant human LRCH2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 372-571 aa of BC125224 Sequence: HSQVSESNREQTSRNDSHIIGSKTDSQKDQEVYDFVDPNTEDVAVPEQGNAHIGSFVSFFKGKEKCSEKSRKNEELGDEKRLEKEQLLAEEEDDDLKEVTDLRKIAAQLLQQEQKNRILNHSTSVMRNKPKQTVECEKSVSADEVNSPLSPLTWQPLENQKDQIDEQPWPESHPIIWQSEERRRSKQIRKEYFKYKSMRK Predict reactive species |
| Full Name | leucine-rich repeats and calponin homology (CH) domain containing 2 |
| Calculated Molecular Weight | 765 aa, 85 kDa |
| Observed Molecular Weight | 84 kDa |
| GenBank Accession Number | BC125224 |
| Gene Symbol | LRCH2 |
| Gene ID (NCBI) | 57631 |
| RRID | AB_2879764 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5VUJ6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Leucine rich repeat and calponin homology domain containing protein 2 (LRCH2) also named as KIAA1495 is a 765 amino acid protein, which contains one CH domain and nine LRR repeats. The function of LRCH2 is still non-known.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for LRCH2 antibody 24866-1-AP | Download protocol |
| WB protocol for LRCH2 antibody 24866-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
