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Lin28B-specific Antibody

Lin28B-specific Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16178-1-AP

Synonyms

CSDD2, lin 28 homolog B (C. elegans), Lin 28B, Lin28, LIN28B, Protein lin 28 homolog B



Tested Applications

Positive IHC detected inhuman testis tissue, human brain tissue, human liver cancer tissue, human lung tissue, human kidney tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

16178-1-AP targets Lin28B-specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name lin-28 homolog B (C. elegans)
Calculated molecular weight 28 kDa
GenBank accession numberNM_001004317
Gene symbol LIN28B
Gene ID (NCBI) 389421
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LIN28B, also named as CSDD2, belongs to the lin-28 family. It acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7). LIN2B is specifically expressed in embryonic muscle, neurons, and epithelia, as well as in embryonic carcinoma cells. However, it is suppressed in most adult tissues except for skeletal and cardiac muscle. LIN2B shares 78% sequence identity with LIN28A. This antibody is specific to LIN28B.

Protocols

Product Specific Protocols
WB protocol for Lin28B-specific antibody 16178-1-APDownload protocol
IHC protocol for Lin28B-specific antibody 16178-1-APDownload protocol
IF protocol for Lin28B-specific antibody 16178-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cell Rep

LIN28B Underlies the Pathogenesis of a Subclass of Ewing Sarcoma LIN28B Control of EWS-FLI1 Stability.

Authors - Tugba Keskin
mouseWB,IHC

Arch Oral Biol

Different expression patterns of Lin28 and Lin28b in mouse molar development.

Authors - Ning Dong
humanIHC

Tumour Biol

Expression and associations of TRAF1, BMI-1, ALDH1, and Lin28B in oral squamous cell carcinoma.

Authors - Tian-Fu Wu
humanWB

Tumour Biol

JMJD1A promotes tumorigenesis and forms a feedback loop with EZH2/let-7c in NSCLC cells.

Authors - Min Zhan
mouseWB,IHC

PLoS One

Unique Haploinsufficient Role of the MicroRNA-Processing Molecule Dicer1 in a Murine Colitis-Associated Tumorigenesis Model.

Authors - Yoshikawa Takeshi T
humanIHC

PLoS One

Increased Expression of Lin28B Associates with Poor Prognosis in Patients with Oral Squamous Cell Carcinoma.

Authors - Tianfu Wu