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MAD2L1 Antibody

MAD2L1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2F5F12

Cat no : 66014-1-Ig

Synonyms

HSMAD2, MAD2, MAD2 like protein 1, MAD2L1



Tested Applications

Positive WB detected inHEK-293 cells, HEK-293
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The 66014-1-Ig immunogen is Fusion Protein MAD2L1 protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MAD2L1 fusion protein Ag17512
Full Name MAD2 mitotic arrest deficient-like 1 (yeast)
Calculated molecular weight 24 kDa
Observed molecular weight 24 kDa
GenBank accession number BC000356
Gene symbol MAD2L1
Gene ID (NCBI) 4085
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1, synonyms: MAD2, HSMAD2) is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is localized to human chromosome band 5q23.3 by in situ hybridization.

Protocols

Product Specific Protocols
WB protocol for MAD2L1 antibody 66014-1-IgDownload protocol
IHC protocol for MAD2L1 antibody 66014-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols