Tested Applications
| Positive WB detected in | A375 cells, A431 cells, U2OS cells, human testis tissue |
| Positive IHC detected in | human lung cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12508-1-AP targets MAGEA4 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3193 Product name: Recombinant human MAGEA4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-317 aa of BC017723 Sequence: MSSEQKSQHCKPEEGVEAQEEALGLVGAQAPTTEEQEAAVSSSSPLVPGTLEEVPAAESAGPPQSPQGASALPTTISFTCWRQPNEGSSSQEEEGPSTSPDAESLFREALSNKVDELAHFLLRKYRAKELVTKAEMLERVIKNYKRCFPVIFGKASESLKMIFGIDVKEVDPTSNTYTLVTCLGLSYDGLLGNNQIFPKTGLLIIVLGTIAMEGDSASEEEIWEELGVMGVYDGREHTVYGEPRKLLTQDWVQENYLEYRQVPGSNPARYEFLWGPRALAETSYVKVLEHVVRVNARVRIAYPSLREAALLEEEEGV Predict reactive species |
| Full Name | melanoma antigen family A, 4 |
| Calculated Molecular Weight | 317 aa, 35 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC017723 |
| Gene Symbol | MAGEA4 |
| Gene ID (NCBI) | 4103 |
| RRID | AB_2877863 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P43358 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MAGEA4, also named MAGE4 and CT1.4, is one of the germ cell-specific markers (PMID:16540528). SMAGEA4 is one of the subgroup I MAGEAs members which may play important roles in the immune surveillance of certain tumor types and play important roles during embryogenesis.(PMID:15222021) The antibody is specific to MAGEA4.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for MAGEA4 antibody 12508-1-AP | Download protocol |
| IHC protocol for MAGEA4 antibody 12508-1-AP | Download protocol |
| WB protocol for MAGEA4 antibody 12508-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MAGEA4 Polyclonal antibody (Cat# 12508-1-AP) has a total of 1 citation. It appears in Frontiers in Endocrinology (Lausanne), published in 2024. The cited study investigated novel mutations in 17β-hydroxysteroid dehydrogenase-3 deficiency and clinical experience with early treatment, placing the research in the fields of endocrinology and genetic disease. The antibody was used for IHC on human samples.
| Species | Application | Title |
|---|---|---|
Front Endocrinol (Lausanne) Four novel mutations identification in 17 beta-hydroxysteroid dehydrogenase-3 deficiency and our clinical experience: possible benefits of early treatment |
