MAGEA4 Polyclonal antibody

MAGEA4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12508-1-AP

Synonyms

Cancer/testis antigen 1.4, CT1.4, MAGE 4 antigen, MAGE 41, MAGE 41 antigen, MAGE X2, MAGE X2 antigen, MAGE4, MAGE4A, MAGE4B, MAGEA4, melanoma antigen family A, 4, Melanoma associated antigen 4



Tested Applications

Positive WB detected inA431 cells, U2OS cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:600-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

12508-1-AP targets MAGEA4 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAGEA4 fusion protein Ag3193
Full Name melanoma antigen family A, 4
Calculated molecular weight 317 aa, 35 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC017723
Gene symbol MAGEA4
Gene ID (NCBI) 4103
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MAGEA4 antibody 12508-1-APDownload protocol
IHC protocol for MAGEA4 antibody 12508-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols