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MAGEH1 Polyclonal antibody

MAGEH1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12424-1-AP

Synonyms

Apoptosis related protein 1, APR 1, APR1, MAGE H1 antigen, MAGEH, MAGEH1, melanoma antigen family H, 1, Melanoma associated antigen H1, Restin

Validation Data Gallery

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  • Western Blot (WB) analysis of Y79 cells using MAGEH1 Polyclonal antibody (12424-1-AP)

    WB analysis of Y79 using 12424-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 12424-1-AP (MAGEH1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using MAGEH1 Polyclonal antibody (12424-1-AP)

    WB analysis of mouse brain using 12424-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12424-1-AP (MAGEH1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using MAGEH1 Polyclonal antibody (12424-1-AP)

    WB analysis of PC-3 using 12424-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12424-1-AP (MAGEH1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MAGEH1 Polyclonal antibody (12424-1-AP)

    IHC staining of human prostate cancer using 12424-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12424-1-AP (MAGEH1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MAGEH1 Polyclonal antibody (12424-1-AP)

    IHC staining of human prostate cancer using 12424-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12424-1-AP (MAGEH1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MAGEH1 Polyclonal antibody (12424-1-AP)

    IHC staining of human prostate cancer using 12424-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12424-1-AP (MAGEH1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using MAGEH1 Polyclonal antibody (12424-1-AP)

    IF Staining of PC-3 using 12424-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 12424-1-AP (MAGEH1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inY79 cells, mouse brain tissue, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12424-1-AP targets MAGEH1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAGEH1 fusion protein Ag3090
Full Name melanoma antigen family H, 1
Calculated Molecular Weight 219 aa, 24 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC011954
Gene Symbol MAGEH1
Gene ID (NCBI) 28986
RRIDAB_2877856
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MAGEH1 antibody 12424-1-APDownload protocol
IHC protocol for MAGEH1 antibody 12424-1-APDownload protocol
IF protocol for MAGEH1 antibody 12424-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Oxid Med Cell Longev

DDAH1/ADMA Regulates Adiponectin Resistance in Cerebral Ischemia via the ROS/FOXO1/APR1 Pathway.

Authors - Yichen Zhao
View Article