LC3 Polyclonal antibody

LC3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish and More (4)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12135-1-AP

Synonyms



Tested Applications

Positive WB detected inChloroquine treated HEK-293 cells, Chloroquine treated HeLa cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inStarvation treated HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12135-1-AP targets LC3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

Tested Reactivity human, mouse, rat, zebrafish
Cited Reactivity Chinese soft-shelled turtles, human, mouse, pig, rat, swine, turtle
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LC3 fusion protein Ag2781
Full Name microtubule-associated protein 1 light chain 3 alpha
Calculated molecular weight 121 aa, 14 kDa
GenBank accession numberBC015810
Gene symbol MAP1LC3A
Gene ID (NCBI) 84557
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LC3, also known as microtubule-associated protein 1 light chain 3 (MAP1LC3), is a mammalian homolog of the yeast Atg8p (autophagy-related 8 protein) that is involved in autophagy process. Three isoforms of LC3 exist as LC3A,LC3B, and LC3C. LC3 is first synthesized as pro-LC3, and undergoes C-terminal cleavage to yield a cytosolic precursor known as LC3-I. During autophagy LC3-I is subsequently conjugated to PE lipid as lipidated form: LC3-II. The lipidated form migrates faster on SDS-PAGE gel. The increased the level of LC3-II has been widely used as autophagy marker. This antibody recognizes both LC3-I (18-20 kDa) and LC3-II (14-16 kDa).

Protocols

Product Specific Protocols
WB protocol for LC3 antibody 12135-1-APDownload protocol
IHC protocol for LC3 antibody 12135-1-APDownload protocol
IF protocol for LC3 antibody 12135-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Evid Based Complement Alternat Med

Tetrandrine-Induced Autophagy in MDA-MB-231 Triple-Negative Breast Cancer Cell through the Inhibition of PI3K/AKT/mTOR Signaling.

Authors - Yubo Guo
turtleIF

Reprod Biol Endocrinol

LIPOPHAGY: a novel form of steroidogenic activity within the LEYDIG cell during the reproductive cycle of turtle.

Authors - Imran Tarique
mouseWB,IF

J Diabetes

Inhibition of HMGB1 attenuates podocyte apoptosis and epithelial-to-mesenchymal transition (EMT) by regulating autophagy flux.

Authors - Juan Jin
mouseWB,IF

Stem Cell Res Ther

Exosome secreted from adipose-derived stem cells attenuates diabetic nephropathy by promoting autophagy flux and inhibiting apoptosis in podocyte.

Authors - Juan Jin
ratWB

J Ethnopharmacol

Mitochondrial dynamics modulation as a critical contribution for Shenmai injection in attenuating hypoxia/reoxygenation injury.

Authors - Jiahui Yu
humanWB

Int J Oncol

α-hederin induces autophagic cell death in colorectal cancer cells through reactive oxygen species dependent AMPK/mTOR signaling pathway activation.

Authors - Jian Sun