Tested Applications
| Positive WB detected in | Recombinant protein |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18726-1-AP targets LC3C-Specific in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | microtubule-associated protein 1 light chain 3 gamma |
| Calculated Molecular Weight | 17 kDa |
| GenBank Accession Number | NM_001004343 |
| Gene Symbol | LC3C |
| Gene ID (NCBI) | 440738 |
| RRID | AB_2878557 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BXW4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LC3C, also named as MAP1LC3C, belongs to the MAP1 LC3 family. It is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. LC3C is produced by the proteolytic cleavage after the conserved C-terminal Gly residue. The antibody is specific to the prepeptide of LC3C.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for LC3C-Specific antibody 18726-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-LC3 antibody (Cat# 18726-1-AP) has 10 total citations published in journals including Autophagy, Redox Biology, MedComm, Developmental Cell, Frontiers in Pharmacology, Journal of Immunology, PLoS ONE, and Advances in Experimental Medicine and Biology. Research fields span autophagy, mitophagy, targeted protein degradation, cGAS-STING signaling, Golgi-selective autophagy, antigen cross-presentation, and cancer biology.
| Species | Application | Title |
|---|---|---|
Autophagy Advancing targeted protein degradation: pLIRTAC's role in glioma and CAR-T cell therapy.
| ||
Autophagy Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy. | ||
Redox Biol Arylsulfatase K attenuates airway epithelial cell senescence in COPD by regulating parkin-mediated mitophagy | ||
MedComm (2020) Dimethyl fumarate alleviate hepatic ischemia-reperfusion injury through suppressing cGAS-STING signaling | ||
Dev Cell TM9SF3 is a Golgi-resident ATG8-binding protein essential for Golgi-selective autophagy | ||
Dev Cell V-ATPase recruitment to ER exit sites switches COPII-mediated transport to lysosomal degradation |
