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MASP1 Polyclonal antibody

MASP1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21837-1-AP

Synonyms

Complement factor MASP 3, CRARF, CRARF1, MASP, MASP 1, MASP1, PRSS5, RaRF, Serine protease 5

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using MASP1 Polyclonal antibody (21837-1-AP)

    WB analysis of HeLa using 21837-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21837-1-AP (MASP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MASP1 Polyclonal antibody (21837-1-AP)

    IHC staining of human liver cancer using 21837-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21837-1-AP (MASP1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MASP1 Polyclonal antibody (21837-1-AP)

    IHC staining of human liver cancer using 21837-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21837-1-AP (MASP1 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21837-1-AP is MASP1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MASP1 fusion protein Ag16432
Full Name mannan-binding lectin serine peptidase 1 (C4/C2 activating component of Ra-reactive factor)
Calculated Molecular Weight 728 aa, 82 kDa
Observed Molecular Weight 40-70 kDa
GenBank Accession NumberBC106945
Gene Symbol MASP1
Gene ID (NCBI) 5648
RRIDAB_2878924
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MASP1 antibody 21837-1-APDownload protocol
IHC protocol for MASP1 antibody 21837-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Front Immunol

Thrombotic microangiopathy mediates poor prognosis among lupus nephritis via complement lectin and alternative pathway activation

Authors - Binshan Zhang
View Article
human,mouseWB,IHC,IP

Hepatology

Hepatocyte-derived MASP1-enriched small extracellular vesicles activate HSCs to promote liver fibrosis

Authors - Xianzhi Liu
  • KD Validated
View Article