MAT2B Antibody

MAT2B Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15952-1-AP

Synonyms

MAT II, MAT II beta, MAT2B, MATIIbeta, Nbla02999, SDR23E1, TGR, UNQ2435/PRO4995



Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, Jurkat cells, mouse brain tissue, mouse testis tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman lung tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 15 publications below

Product Information

The 15952-1-AP immunogen is Fusion Protein MAT2B protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity chicken, hen, hens, human, Landrace, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAT2B fusion protein Ag8649
Full Name methionine adenosyltransferase II, beta
Calculated molecular weight 36 kDa;38 kDa
Observed molecular weight 38 kDa
GenBank accession number BC005218
Gene symbol MAT2B
Gene ID (NCBI) 27430
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MAT2B antibody 15952-1-APDownload protocol
IHC protocol for MAT2B antibody 15952-1-APDownload protocol
IF protocol for MAT2B antibody 15952-1-APDownload protocol
IP protocol for MAT2B antibody 15952-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

J Agric Food Chem

Maternal Betaine Supplementation during Gestation Enhances Expression of mtDNA-Encoded Genes through D-Loop DNA Hypomethylation in the Skeletal Muscle of Newborn Piglets.

Authors - Yimin Jia
ratWB

J Agric Food Chem

Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams.

Authors - Shu Yang
chickenWB

Comp Biochem Physiol B Biochem Mol Biol

Maternal betaine administration modulates hepatic type 1 iodothyronine deiodinase (Dio1) expression in chicken offspring through epigenetic modifications.

Authors - Zhen Hou
human

Ann N Y Acad Sci

PMID: 29722030

Authors - Holly M Brown-Borg
humanWB

Poult Sci

Prenatal betaine exposure modulates hypothalamic expression of cholesterol metabolic genes in cockerels through modifications of DNA methylation.

Authors - Abdulrahman A Idriss
hensWB

Poult Sci

Maternal betaine supplementation decreases hepatic cholesterol deposition in chicken offspring with epigenetic modulation of SREBP2 and CYP7A1 genes.

Authors - Yun Hu