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MDH1 Antibody

MDH1 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

IF, WB, ELISA

Conjugate

Unconjugated

Publications

0

CloneNo.

1F9A2

Cat no : 66505-1-Ig

Synonyms

Cytosolic malate dehydrogenase, MDH s, MDH1, MDHA, MOR2



Tested Applications

Positive WB detected inHeLa cell, HEK-293 cell, Jurkat cell, HeLa cells, NIH/3T3 cells, RAW 264.7 cells, HEK-293 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2500-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66505-1-Ig targets MDH1 in IF, WB, ELISA applications and shows reactivity with Human, mouse samples.

Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MDH1 fusion protein Ag8744
Full Name malate dehydrogenase 1, NAD (soluble)
Calculated molecular weight 334aa,36 kDa
Observed molecular weight 36-37 kDa
GenBank accession number BC001484
Gene symbol MDH1
Gene ID (NCBI) 4190
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

MDH1(Malate dehydrogenase, cytoplasmic) is also named as MDHA and belongs to the LDH/MDH superfamily and MDH type 2 family which catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. It can exsit as a dimer and the dimeric MDH1 is the mitochondrial isoenzyme, whereas the tetrameric MDH2 is the glycosomal isoenzyme.(PMID:10693743).

Protocols

Product Specific Protocols
WB protocol for MDH1 antibody 66505-1-IgDownload protocol
IF protocol for MDH1 antibody 66505-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols