Tested Applications
| Positive IHC detected in | human endometrial cancer tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
51080-1-AP targets MEK1-Specific in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, eriocheir sinensis |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | mitogen-activated protein kinase kinase 1 |
| Calculated Molecular Weight | 43 kDa |
| GenBank Accession Number | BC139729 |
| Gene Symbol | MEK1 |
| Gene ID (NCBI) | 5604 |
| ENSEMBL Gene ID | ENSG00000169032 |
| RRID | AB_2881242 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q02750 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MEK1 is also named as MAP2K1 (mitogen-activated protein kinase kinase 1), MAPKK1, PRKMK1, MKK1 and belongs to the MAP kinase kinase subfamily. It is a dual-specificity kinase that mediate ERK1 and ERK2 activation during adhesion and growth factor signaling(PMID:19219045). It also plays an essential role in extra-embryonic ectoderm during placentogenesis. MEK1 has 2 isoforms produced by alternative splicing with the molecular weight of 43 kDa and 41 kDa. It can be phosphorylated(PMID:21144847). This antibody is specific to MEK1.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MEK1-Specific antibody 51080-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MEK1-Specific Polyclonal antibody (Cat# 51080-1-AP) has 8 citations spanning journals including Front Med, Cancer Cell Int, J Ethnopharmacol, Mol Cell Probes, Comp Biochem Physiol D, PLoS One, and Onco Targets Ther. Research fields cover hepatocellular carcinoma, cholangiocarcinoma, glioblastoma, colorectal cancer, preeclampsia, ovarian transcriptomics, eIF5B-mediated proteostasis, and lung adenocarcinoma, with most studies utilizing WB in human samples.
| Species | Application | Title |
|---|---|---|
Front Med 5'-tiRNA-Gln inhibits hepatocellular carcinoma progression by repressing translation through the interaction with eukaryotic initiation factor 4A-I | ||
Cancer Cell Int 4,5-Dimethoxycanthin-6-one is a novel LSD1 inhibitor that inhibits proliferation of glioblastoma cells and induces apoptosis and pyroptosis. | ||
Cancer Cell Int MiR-155-5p suppresses SOX1 to promote proliferation of cholangiocarcinoma via RAF/MEK/ERK pathway. | ||
J Ethnopharmacol Classical prescription Floris Sophorae Powder treat colorectal cancer by regulating KRAS/MEK-ERK signaling pathway | ||
Mol Cell Probes Elevated PGT promotes proliferation and inhibits cell apoptosis in preeclampsia by Erk signaling pathway | ||
Comp Biochem Physiol Part D Genomics Proteomics Effects of dietary icariin supplementation on the ovary development-related transcriptome of Chinese mitten crab (Eriocheir sinensis). |
