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MEX3C Polyclonal antibody

MEX3C Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22882-1-AP

Synonyms

BM 013, mex 3 homolog C (C. elegans), MEX 3C, MEX3C, RING finger protein 194, RKHD2, RNA binding protein MEX3C, RNF194

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using MEX3C Polyclonal antibody (22882-1-AP)

    WB analysis of HeLa using 22882-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22882-1-AP (MEX3C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using MEX3C Polyclonal antibody (22882-1-AP)

    WB analysis of PC-3 using 22882-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 22882-1-AP (MEX3C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using MEX3C Polyclonal antibody (22882-1-AP)

    IHC staining of human testis using 22882-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22882-1-AP (MEX3C Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MEX3C Polyclonal antibody (22882-1-AP)

    IHC staining of human testis using 22882-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22882-1-AP (MEX3C Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MEX3C Polyclonal antibody (22882-1-AP)

    IF Staining of HeLa using 22882-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 22882-1-AP (MEX3C antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, PC-3 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22882-1-AP targets MEX3C in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MEX3C fusion protein Ag18931
Full Name mex-3 homolog C (C. elegans)
Calculated Molecular Weight 659 aa, 69 kDa
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC148855
Gene Symbol MEX3C
Gene ID (NCBI) 51320
RRIDAB_2879183
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MEX3C(RNA-binding E3 ubiquitin-protein ligase MEX3C) is also named as RKHD2, RNF194 and may be involved in post-transcriptional regulatory mechanisms. MEX3C is necessary for normal postnatal growth and enhances the expression of local bone Igf1 expression(PMID:22927639). This protein is expressed in the cytoplasm and shuttles between the cytoplasm and the nucleus through the CRM1 export pathway. At least three MEX3C protein variants can be expressed by alternative splicing and alternative transcription initiation: MEX3C659AA(containing 659 amino acid residues, about 82 kDa), MEX3C464AA(about 64 kDa) and MEX3C372AA(about 50 kDa)(PMID: 28982131,27053362,22863774).

Protocols

Product Specific Protocols
WB protocol for MEX3C antibody 22882-1-APDownload protocol
IHC protocol for MEX3C antibody 22882-1-APDownload protocol
IF protocol for MEX3C antibody 22882-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Gastroenterology

A High-Content Screen Identifies MicroRNAs That Regulate Liver Repopulation After Injury in Mice.

Authors - Adam M Zahm
View Article
humanWB,IF

Biol Reprod

Oocyte-Specific Expression of Mouse MEX3C652AA in the Ovary and Its Potential Role in Regulating Maternal Fos mRNA.

Authors - Xue Li
View Article