MFF Monoclonal antibody

MFF Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G4B12

Cat no : 66527-1-Ig

Synonyms

AD033, C2orf33, MFF, mitochondrial fission factor



Tested Applications

Positive WB detected inpig brain tissue, HepG2 cells, L02 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

66527-1-Ig targets MFF in WB, IHC, ELISA applications and shows reactivity with Human, pig, rat, mouse samples.

Tested Reactivity Human, pig, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MFF fusion protein Ag9990
Full Name mitochondrial fission factor
Calculated molecular weight 38 kDa
Observed molecular weight 26-29 kDa, 35-38 kDa
GenBank accession numberBC000797
Gene symbol MFF
Gene ID (NCBI) 56947
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MFF (mitochondrial fission factor) is a mitochondrial outer membrane protein that is involved in mitochondrial localization of Drp1 and mitochondrial fission. Multiple isoforms of MFF exist due to the alternative splicing. This antibody recognizes the endogenous MFF protein around 26-29 kDa and 35-38 kDa.

Protocols

Product Specific Protocols
WB protocol for MFF antibody 66527-1-IgDownload protocol
IHC protocol for MFF antibody 66527-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

JAE HO (Verified Customer) (05-06-2019)

25 ug of PC3 cell lysates were used for western blot. 500 ug of cell lysates and 2.5 ug of antibodies were used for IP. Comparing to "17090-1-AP", it looks less titer but it works well for WB and IP.

  • Applications: Western Blot,Immunoprecipitation,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: PC3 cell