MLKL Antibody

MLKL Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21066-1-AP

Synonyms

BC028141, MLKL



Tested Applications

Positive WB detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The 21066-1-AP immunogen is Fusion Protein MLKL protein expressed in E. coli.

Reactivity human
Cited Species human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MLKL fusion protein Ag15188
Full Name mixed lineage kinase domain-like
Calculated molecular weight 471aa,54 kDa
Observed molecular weight 54 kDa
GenBank accession number BC028141
Gene symbol MLKL
Gene ID (NCBI) 197259
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mixed lineage kinase domain like pseudokinase (MLKL), belongs to the protein kinase superfamily, has two MW of 54 and 30 kDa. MLKL plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children.

Protocols

Product Specific Protocols
WB protocol for MLKL antibody 21066-1-APDownload protocol
IHC protocol for MLKL antibody 21066-1-APDownload protocol
IF protocol for MLKL antibody 21066-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cardiovasc Drugs Ther

Dexmedetomidine Preconditioning Protects Cardiomyocytes Against Hypoxia/Reoxygenation-Induced Necroptosis by Inhibiting HMGB1-Mediated Inflammation.

Authors - Jingyi Chen
mouseWB

J Cell Sci

FKBP12 mediates necroptosis by initiating RIPK1/RIPK3/MLKL signal transduction in response to TNF receptor 1 ligation.

Authors - Zicheng Wang
mouseWB

Arch Immunol Ther Exp (Warsz)

Visfatin Plays a Significant Role in Alleviating Lipopolysaccharide-Induced Apoptosis and Autophagy Through PI3K/AKT Signaling Pathway During Acute Lung Injury in Mice.

Authors - Xin-Tong Wu
mouseWB

Toxicol Appl Pharmacol

Dihydrotanshinone I, a natural product, ameliorates DSS-induced experimental ulcerative colitis in mice.

Authors - Yanling Guo
mouseWB

Am J Chin Med

Neferine, a Bisbenzylisoquinoline Alkaloid, Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis.

Authors - Xiaxia Wu
humanWB,IP

Cell Death Dis

A necroptotic-independent function of MLKL in regulating endothelial cell adhesion molecule expression.

Authors - Jialin Dai