|Positive WB detected in||HepG2 cells|
|Positive IHC detected in||human liver cancer tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 21066-1-AP is MLKL Fusion Protein expressed in E. coli.
|Host / Isotype||Rabbit / IgG|
|Immunogen||MLKL fusion protein Ag15188|
|Full Name||mixed lineage kinase domain-like|
|Calculated molecular weight||471 aa, 54 kDa|
|Observed molecular weight||54 kDa|
|GenBank accession number||BC028141|
|Gene ID (NCBI)||197259|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Mixed lineage kinase domain like pseudokinase (MLKL), belongs to the protein kinase superfamily, has two MW of 54 and 30 kDa. MLKL plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children.
Cardiovasc Drugs Ther
J Cell Sci
FKBP12 mediates necroptosis by initiating RIPK1/RIPK3/MLKL signal transduction in response to TNF receptor 1 ligation.
Arch Immunol Ther Exp (Warsz)
Visfatin Plays a Significant Role in Alleviating Lipopolysaccharide-Induced Apoptosis and Autophagy Through PI3K/AKT Signaling Pathway During Acute Lung Injury in Mice.
Toxicol Appl Pharmacol
Dihydrotanshinone I, a natural product, ameliorates DSS-induced experimental ulcerative colitis in mice.
Am J Chin Med
Neferine, a Bisbenzylisoquinoline Alkaloid, Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis.
Cell Death Dis
A necroptotic-independent function of MLKL in regulating endothelial cell adhesion molecule expression.