Tested Applications
| Positive WB detected in | BxPC-3 cells, HeLa cells, HepG2 cells, L02 cells, DU 145 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human ovarian cancer, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11137-1-AP targets MMAB in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1602 Product name: Recombinant human MMAB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-250 aa of BC011831 Sequence: MAVCGLGSRLGLGSRLGLRGCFGAARLLYPRFQSRGPQGVEDGDRPQPSSKTPRIPKIYTKTGDKGFSSTFTGERRPKDDQVFEAVGTTDELSSAIGFALELVTEKGHTFAEELQKIQCTLQDVGSALATPCSSAREAHLKYTTFKAGPILELEQWIDKYTSQLPPLTAFILPSGGKISSALHFCRAVCRRAERRVVPLVQMGETDANVAKFLNRLSDYLFTLARYAAMKEGNQEKIYMKNDPSAESEGL Predict reactive species |
| Full Name | methylmalonic aciduria (cobalamin deficiency) cblB type |
| Calculated Molecular Weight | 27 kDa |
| Observed Molecular Weight | 27 kDa |
| GenBank Accession Number | BC011831 |
| Gene Symbol | MMAB |
| Gene ID (NCBI) | 326625 |
| RRID | AB_2235498 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96EY8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The MMAB gene encodes cob(I)alamin adenosyltransferase, which catalyzes the final step in the synthesis of the coenzyme adenosylcobalamin (AdoCbl). AdoCbl is a vitamin B12-containing coenzyme for methylmalonyl-CoA mutase. The deduced 250-amino acid protein has a calculated molecular mass of 27.3 kD.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MMAB antibody 11137-1-AP | Download protocol |
| IHC protocol for MMAB antibody 11137-1-AP | Download protocol |
| IP protocol for MMAB antibody 11137-1-AP | Download protocol |
| WB protocol for MMAB antibody 11137-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MMAB Polyclonal antibody (Cat# 11137-1-AP) has 4 citations. These appear in Biochim Biophys Acta Mol Basis Dis, Biochim Biophys Acta, Hum Mol Genet, and Hum Mutat. The research fields center on methylmalonic aciduria cblB type, pharmacological chaperoning therapy, induced pluripotent stem cell differentiation into hepatocyte-like cells, and functional/structural analysis of MMAB gene mutations.
| Species | Application | Title |
|---|---|---|
Biochim Biophys Acta Mol Basis Dis Hepatocyte-like cells differentiated from methylmalonic aciduria cblB type induced pluripotent stem cells: A platform for the evaluation of pharmacochaperoning. | ||
Biochim Biophys Acta New perspectives for pharmacological chaperoning treatment in methylmalonic aciduria cblB type. | ||
Hum Mol Genet Pharmacological chaperones as a potential therapeutic option in methylmalonic aciduria cblB type. | ||
Hum Mutat Functional and structural analysis of five mutations identified in methylmalonic aciduria cblB type. |
