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MMP9 (N-terminal) Polyclonal antibody

MMP9 (N-terminal) Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10375-2-AP

Synonyms

92 kDa gelatinase, 92 kDa type IV collagenase, CLG4B, Gelatinase B, GELB, Matrix metalloproteinase 9, MMP 9, MMP9



Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, A375 cells, rat brain tissue, Raji cells, HUVEC cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman breast cancer tissue, human cervical cancer tissue, human tonsillitis tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10375-2-AP targets MMP9 (N-terminal) in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity Astragalus membranaceus, hamster, human, mouse, rabbit, rat, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MMP9 (N-terminal) fusion protein Ag0552
Full Name matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
Calculated molecular weight 707 aa, 78 kDa
Observed molecular weight 67 kDa, 92 kDa
GenBank accession numberBC006093
Gene symbol MMP9
Gene ID (NCBI) 4318
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. Matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) (MMP9, synonyms: GELB, CLG4B) degrades collagens type IV and V. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. The pro-MMP9 is 92 kDa, and it can be detected a processed form of 68 kDa. This protein can exist as a dimer of 180 kDa (PMID:7492685).

Protocols

Product Specific Protocols
WB protocol for MMP9 (N-terminal) antibody 10375-2-APDownload protocol
IHC protocol for MMP9 (N-terminal) antibody 10375-2-APDownload protocol
IF protocol for MMP9 (N-terminal) antibody 10375-2-APDownload protocol
IP protocol for MMP9 (N-terminal) antibody 10375-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Brain Behav Immun

Magnolol exhibits anti-inflammatory and neuroprotective effects in a rat model of intracerebral haemorrhage.

Authors - Fangfang Zhou
mouseIF

JCI Insight

Matrix metalloproteinase-9 deficiency protects mice from severe influenza A viral infection.

Authors - Joselyn Rojas-Quintero
humanWB

Mol Cancer

Alteration of tumor suppressor BMP5 in sporadic colorectal cancer: a genomic and transcriptomic profiling based study.

Authors - Erfei Chen
humanWB

Cytotechnology

Angiopoietin-like protein 1 inhibits epithelial to mesenchymal transition in colorectal cancer cells via suppress Slug expression.

Authors - Haijun Fan
mouseWB

Dose Response

Low-Dose Radiation Enhanced Inhibition of Breast Tumor Xenograft and Reduced Myocardial Injury Induced by Doxorubicin.

Authors - Xin Jiang
humanWB

J Cell Physiol

Rg3 inhibits gemcitabine-induced lung cancer cell invasiveness through ROS-dependent, NF-κB- and HIF-1α-mediated downregulation of PTX3.

Authors - Bulbul Ahmmed

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kyosuke (Verified Customer) (06-12-2019)

I am working on aneurysm study and use this for IHC staining on mouse aneurysm section. It works very well.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1/100
  • Cell Tissue Type: mouse aorta
FH

JIMMY (Verified Customer) (04-15-2019)

This antibody detects MMP9 expressing cells in the spinal cord of C57BL/6 EAE mice. The imaging is quiet clear with this antibody.

  • Applications: Immunofluorescence,
FH

Lucy (Verified Customer) (03-08-2019)

Reacts mainly to human MMP9 but slightly cross-reactive to human MMP2

  • Applications: Western Blot,
MMP9 (N-terminal) Antibody Western Blot, validation ( dilution) in (Cat no:10375-2-AP)