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MRPL19 Polyclonal antibody

MRPL19 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16517-1-AP

Synonyms

KIAA0104, L15mt, L19mt, MRP L15, MRP L19, MRPL15, MRPL19, RLX1, RPML15

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using MRPL19 Polyclonal antibody (16517-1-AP)

    WB analysis of mouse kidney using 16517-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16517-1-AP (MRPL19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MRPL19 Polyclonal antibody (16517-1-AP)

    WB analysis of HeLa using 16517-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16517-1-AP (MRPL19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using MRPL19 Polyclonal antibody (16517-1-AP)

    WB analysis of mouse liver using 16517-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16517-1-AP (MRPL19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MRPL19 Polyclonal antibody (16517-1-AP)

    IF Staining of HeLa using 16517-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16517-1-AP (MRPL19 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse kidney tissue, HeLa cells, mouse liver tissue
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

16517-1-AP targets MRPL19 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPL19 fusion protein Ag9738
Full Name mitochondrial ribosomal protein L19
Calculated Molecular Weight 292 aa, 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC030144
Gene Symbol MRPL19
Gene ID (NCBI) 9801
RRIDAB_2250806
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MRPL19 antibody 16517-1-APDownload protocol
IF protocol for MRPL19 antibody 16517-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Transl Lung Cancer Res

Overexpression of MRPL19 in predicting poor prognosis and promoting the development of lung adenocarcinoma

Authors - Dong Wei
View Article