MRPS27 Monoclonal antibody

MRPS27 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B1B3

Cat no : 66724-1-Ig

Synonyms

FLJ21764, FLJ23348, KIAA0264, MRP S27, MRPS27, S27mt



Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, A431 cells, HepG2 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

66724-1-Ig targets MRPS27 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MRPS27 fusion protein Ag11047
Full Name mitochondrial ribosomal protein S27
Calculated molecular weight 168 aa, 19 kDa, 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC030521
Gene symbol MRPS27
Gene ID (NCBI) 23107
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Genes encoding PPR (pentatricopeptide repeat)-containing proteins constitute one of the largest gene families in plants. The majority of these proteins are predicted to target organelles and to bind to RNA. Strikingly, there is a dearth of these proteins in mammals, although genomic searches reveal six candidates, all of which are also predicted to target the mitochondrion. One of these proteins, MRPS27is participated in transcription and translation respectively. [PMID:18939947] MRPS27is a component of the small subunit of the mitochondrial ribosome that is encoded by the nuclear genome [PMID: 11279123].

Protocols

Product Specific Protocols
WB protocol for MRPS27 antibody 66724-1-IgDownload protocol
IHC protocol for MRPS27 antibody 66724-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Aging (Albany NY)

Qualitative and quantitative alterations in intracellular and membrane glycoproteins maintain the balance between cellular senescence and human aging.

Authors - Yoko Itakura