MRPS27 Monoclonal antibody, PBS Only

MRPS27 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B1B3

Cat no : 66724-1-PBS

Synonyms

FLJ21764, FLJ23348, KIAA0264, MRP S27, MRPS27, S27mt

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Validation Data Gallery

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  WB analysis of LNCaP using 66724-1-Ig (same clone as 66724-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66724-1-Ig (MRPS27 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66724-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66724-1-Ig (same clone as 66724-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66724-1-Ig (MRPS27 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66724-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 66724-1-Ig (same clone as 66724-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66724-1-Ig (MRPS27 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66724-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66724-1-Ig (same clone as 66724-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66724-1-Ig (MRPS27 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66724-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66724-1-Ig (same clone as 66724-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66724-1-Ig (MRPS27 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66724-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66724-1-Ig (same clone as 66724-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66724-1-Ig (MRPS27 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66724-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66724-1-PBS targets MRPS27 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: MRPS27 fusion protein Ag11047
• Full Name: mitochondrial ribosomal protein S27
• Calculated Molecular Weight: 168 aa, 19 kDa, 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC030521
• Gene Symbol: MRPS27
• Gene ID (NCBI): 23107
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Genes encoding PPR (pentatricopeptide repeat)-containing proteins constitute one of the largest gene families in plants. The majority of these proteins are predicted to target organelles and to bind to RNA. Strikingly, there is a dearth of these proteins in mammals, although genomic searches reveal six candidates, all of which are also predicted to target the mitochondrion. One of these proteins, MRPS27is participated in transcription and translation respectively. [PMID:18939947] MRPS27is a component of the small subunit of the mitochondrial ribosome that is encoded by the nuclear genome [PMID: 11279123].